Determination of Malachite Green in Aquaculture Water by Adsorptive Stripping Voltammetry
Determination of Malachite Green in Aquaculture Water by Adsorptive Stripping Voltammetry
复制标题
吸附溶出伏安法测定养殖水中孔雀石绿
DOI：
10.1080/00032719.2015.1104321
复制
发表时间：
2016-05
影响因子：
2
通讯作者：
Chen Gang
中科院分区：
文献类型：
--
作者：
Zhu Dan;Li Qiangqiang;Honeychurch Kevin C.;Piano Martina;Chen Gang
建立了吸附溶出伏安法测定水产养殖水中孔雀石绿的方法。初步研究了孔雀石绿在0.1 M磷酸盐缓冲液（pH 2 ~ 10）上的玻碳电极上的循环伏安法。孔雀石绿及其还原产物白垩孔雀石绿的表征证实了孔雀石绿的氧化还原行为。在pH为7.4时，白孔雀石绿对孔雀石绿的测定无干扰。因此，对吸附溶出伏安法测定水产养殖水中孔雀石绿进行了进一步的研究。优化了伏安波形、积累电位和积累时间。差分脉冲伏安法测定孔雀石绿在0.2µM ~ 1.2µM范围内呈线性关系，灵敏度为0.8311µa /µM。采用多次标准添加法，添加0.5µM和0.75µM孔雀石绿的水产养殖水的平均回收率分别为78.79%和87.20%，变异系数分别为2.07%和1.45%。分析结果表明，该方法可快速、简便、经济、准确地测定水产养殖水体中的孔雀石绿。
ABSTRACT An adsorptive stripping voltammetric method for the determination of malachite green in aquaculture water has been developed. Initial studies were made using the cyclic voltammetry of malachite green at a glassy carbon electrode in 0.1 M phosphate buffer from pH 2 to 10. The redox behavior observed for malachite green was verified by the characterization of malachite green and its reduction product, leucomalachite green. Furthermore, leucomalachite green was found not to interfere with the determination of malachite green at pH 7.4, the optimum pH for malachite green determination. As a result, further studies were performed using adsorptive stripping voltammetry for the determination of malachite green in aquaculture water. The voltammetric waveform, accumulation potential, and accumulation time were optimized. The calibration plot was linear from 0.2 µM to 1.2 µM for malachite green using differential pulse voltammetry with a sensitivity of 0.8311 µA/µM. Using the method of multiple standard addition, aquaculture water fortified with 0.5 µM and 0.75 µM malachite green provided mean recoveries of 78.79% and 87.20% with coefficients of variation of 2.07% and 1.45%. Therefore, analytical figures of merit suggest that this method provides rapid, simple, economical, and precise determination of malachite green in aquaculture water.
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DOI：
10.4236/ajac.2012.33027
发表时间：
2012-03
期刊：
American Journal of Analytical Chemistry
影响因子：
--
作者：
N. Cuong;S. Ishizaka;N. Kitamura
通讯作者：
N. Cuong;S. Ishizaka;N. Kitamura
影响因子：
15
作者：
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通讯作者：
Z. Galus;R. Adams
影响因子：
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作者：
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通讯作者：
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DOI：
10.1016/s1452-3981(23)15160-1
发表时间：
2009-04
影响因子：
1.5
作者：
Liqin Liu;Faqiong Zhao;Fei Xiao;Baizhao Zeng
通讯作者：
Liqin Liu;Faqiong Zhao;Fei Xiao;Baizhao Zeng
影响因子：
3
作者：
Reza Sahraei;Abbas Farmany;S. S. Mortazavi-S.;H. Noorizadeh
通讯作者：
Reza Sahraei;Abbas Farmany;S. S. Mortazavi-S.;H. Noorizadeh