Quantitative neoplastic transformation of C3H/10T1/2 fibroblasts: dependence upon the size of the initiated cell colony at confluence.
Quantitative neoplastic transformation of C3H/10T1/2 fibroblasts: dependence upon the size of the initiated cell colony at confluence.
复制标题
C3H/10T1/2 成纤维细胞的定量肿瘤转化：取决于汇合时起始细胞集落的大小。
作者：
L. Mordan;J. E. Martner;J. Bertram
化学或物理致癌剂对C3H/10T1/2成纤维细胞的转化效率与细胞的接种密度成反比。汇合时的集落大小、累积的细胞世代以及与接种密度无关的表观遗传事件已被提出来解释这种变异性。通过控制汇合时起始细胞的总数和集落大小（即细胞/集落）及其累积的细胞世代，我们确定（a）汇合时起始细胞的集落大小与转化表型的表达直接相关，（b）起始细胞形成转化细胞集落的概率不随大量细胞世代的积累而变化，以及（c）汇合培养物中起始细胞的总数并不决定转化的数量病灶形成。我们开发了一种数学表示，当应用于我们的结果和其他实验室发表的结果时，可以清楚地描述汇合时起始细胞集落的大小与转化表型的表达之间的关系。这些结果对于化学和物理致癌物对 C3H/10T1/2 细胞转化的定量具有重要意义。
The efficiency of transformation of C3H/10T1/2 fibroblasts by chemical or physical carcinogens varies inversely with the seeding density of the cells. Colony size at confluence, accumulated cell generations, and epigenetic events independent of seeding density have been proposed to explain this variability. By controlling the total number and colony size (i.e., cells/colony) of initiated cells at confluence and their accumulated cell generations, we have determined that (a) the colony size of the initiated cells at confluence is directly related to the expression of the transformed phenotype, (b) the probability that an initiated cell will form a colony of transforming cells does not vary with the accumulation of large numbers of cell generations, and (c) the total number of initiated cells in a confluent culture does not determine the number of transformed foci formed. We have developed a mathematical representation which, when applied to our results and the published results from other laboratories, clearly describes the relationship between the size of the initiated cell colony at confluence and the expression of the transformed phenotype. These results have significant implications in the quantification of the transformation of C3H/10T1/2 cells by chemical and physical carcinogens.