Fatty acid desaturation in red blood cell membranes of patients with type 2 diabetes is improved by zinc supplementation
Fatty acid desaturation in red blood cell membranes of patients with type 2 diabetes is improved by zinc supplementation
复制标题
补锌改善2型糖尿病患者红细胞膜脂肪酸脱饱和度
DOI：
10.1016/j.jtemb.2020.126571
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发表时间：
2020-12-01
影响因子：
3.5
通讯作者：
Ruz, Manuel
中科院分区：
文献类型：
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作者：
Catalina Hernandez, Maria;Rojas, Pamela;Ruz, Manuel
背景/目的：膜弹性可能是2型糖尿病（T2 D）病理生理机制的决定性因素。锌作为δ-5去饱和酶（D5 D）和δ-6去饱和酶（D 6D）的辅因子和基因表达调节因子，可能通过增加膜多不饱和脂肪酸（PUFA）的丰度来调节膜的柔韧性。本研究的目的是评估24个月的补锌（30毫克元素锌）对T2D.Subjects/Methods患者膜脂肪酸组成的影响：60例T2 D患者进行了评价。30名被随机分配到锌补充组和30名安慰剂组。用气相色谱法测定红细胞膜脂肪酸组成。结果：补充24个月后，发现二十二碳五烯酸（C22：5 n-3）、花生四烯酸（C20：4 n-6）、肾上腺酸（C22：4 n-6）和总n-6多不饱和脂肪酸（n-6 PUFA）的丰度更高（分别为p = 0.001，p = 0.007，p = 0.033，p = 0.048）。在第24个月，锌补充组的不饱和脂肪酸/饱和脂肪酸比率和不饱和指数增加（分别为p = 0.003和p = 0.000）。FADS 1基因在锌组与安慰剂在第12个月上调（p = 0.020）。结论：补充30 mg/d元素锌在24个月的T2 D患者的红细胞膜的组成增加PUFA丰度，反过来，提高膜的灵活性的影响。这种作用可能是通过诱导D5 D基因表达介导的。
Background/Objective: Membrane flexibility can be a determining factor in pathophysiological mechanisms of type 2 diabetes (T2D). As a cofactor of delta-5 desaturase (D5D) and delta-6 desaturase (D6D), and gene expression regulator, zinc may play a role modulating membrane flexibility by increasing membrane polyunsaturated fatty acids (PUFA) abundance. The objective of this study was to evaluate the effect of a 24-month zinc supplementation (30 mg elemental zinc) on membrane fatty acid composition in patients with T2D.Subjects/methods: Sixty patients with T2D were evaluated. Thirty were randomly assigned to the zinc supplemented group and thirty to the placebo group. Fatty acid composition in red blood cell (RBC) membranes was determined by gas chromatography. Expression of gene encoding for D5D (FADS1), and D6D (FADS2) were evaluated in peripheral blood mononuclear cells by real-time polymerase chain reaction.Results: After 24 months of supplementation, a greater abundance of docosapentaenoic acid (C22:5 n-3), arachidonic acid (C20:4 n-6), adrenic acid (C22:4 n-6), and total n-6 PUFA was found (p = 0.001, p = 0.007, p = 0.033, p = 0.048, respectively). The unsaturated fatty acids/saturated fatty acids ratio, and unsaturation index was increased in the zinc supplemented group at month 24 (p = 0.003 and p = 0.000, respectively). FADS1 gene was upregulated in the zinc group in relation to placebo at month 12 (p = 0.020).Conclusions: Supplementation with 30 mg/d elemental zinc during 24 months in patients with T2D had an effect on the composition of RBC membranes increasing PUFA abundance and in turn, improving membrane flexibility. This effect may be mediated by induction of D5D gene expression.