Diversity and dynamics of microbial communities in brown planthopper at different developmental stages revealed by high-throughput amplicon sequencing
Diversity and dynamics of microbial communities in brown planthopper at different developmental stages revealed by high-throughput amplicon sequencing
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高通量扩增子测序揭示褐飞虱不同发育阶段微生物群落的多样性和动态
DOI：
10.1111/1744-7917.12729
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发表时间：
--
期刊：
影响因子：
4
通讯作者：
Yu XP
中科院分区：
文献类型：
--
作者：
Wang ZL;Wang TZ;Zhu HF;Pan HB;Yu XP
褐飞虱（BPH）相关的微生物组在介导宿主健康和适应性方面起着重要作用。微生物群落及其结构的表征是了解微生物与寄主昆虫之间复杂共生关系的前提。在这里，我们使用高通量扩增子测序研究了不同发育阶段BPH的细菌和真菌群落。我们的研究结果表明，无论是细菌和真菌群落的多样性和动态的BPH的发展。细菌群落在各发育阶段均比真菌丰富。在97%的相似性，19门和278属的细菌进行了注释，而5个真菌门，包括80属被分配。若虫期细菌群落的物种丰富度最高。与其他发育阶段相比，雌成虫真菌群落的分类多样性总体上处于较高水平。各发育阶段细菌和真菌的优势类群均为变形菌门和子囊菌门。与其他发育阶段相比，卵期的细菌属Acinetoacidum的丰度显著较低，而优势真菌属Wallemia在若虫和成虫阶段比卵期更丰富。此外，男性和女性成年人之间的微生物组成不同，表明BPH中的微生物群落具有性别依赖性。总的来说，我们的研究丰富了我们对与BPH相关的微生物群落的知识，并将为开发针对这种水稻害虫的潜在生物防治技术提供线索。
The microbiome associated with brown planthopper (BPH) plays an important role in mediating host health and fitness. Characterization of the microbial community and its structure is prerequisite for understanding the intricate symbiotic relationships between microbes and host insect. Here, we investigated the bacterial and fungal communities of BPH at different developmental stages using high‐throughput amplicon sequencing. Our results revealed that both the bacterial and fungal communities were diverse and dynamic during BPH development. The bacterial communities were generally richer than fungi in each developmental stage. At 97% similarly, 19 phyla and 278 genera of bacteria were annotated, while five fungal phyla comprising 80 genera were assigned. The highest species richness for the bacterial communities was detected in the nymphal stage. The taxonomic diversity of the fungal communities in female adults was generally at a relatively higher level when compared to other developmental stages. The most dominant phylum of bacteria and fungi at each developmental stage all belonged to Proteobacteria and Ascomycota, respectively. A significantly lower abundance of bacterial genusAcinetobacterwas recorded in the egg stage when compared to other developmental stages, while the dominant fungal genusWallemiawas more abundant in the nymph and adult stages than in the egg stage. Additionally, the microbial composition differed between male and female adults, suggesting that the microbial communities in BPH were gender‐dependent. Overall, our study enriches our knowledge on the microbial communities associated with BPH and will provide clues to develop potential biocontrol techniques against this rice pest.