Antimicrobial susceptibility of Enterococcus faecalis isolated from canals of root filled teeth with periapical lesions
Antimicrobial susceptibility of Enterococcus faecalis isolated from canals of root filled teeth with periapical lesions
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DOI：
10.1111/j.1365-2591.2004.00865.x
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发表时间：
2004-11-01
影响因子：
5
通讯作者：
Souza, FJ
中科院分区：
文献类型：
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作者：
Pinheiro, ET;Gomes, BPFA;Souza, FJ
目的检测从根尖周病患者根管中分离的粪肠球菌对不同抗生素的体外敏感性。从具有持续根尖周病变的根填充牙齿的根管中分离的粪菌被测试了它们的抗生素敏感性。使用了以下抗生素：苄青霉素、阿莫西林、阿莫西林-克拉维酸、红霉素、阿奇霉素、万古霉素、氯霉素、四环素、多西环素、环丙沙星和阿托沙星。使用E-test系统（AB BIODISK，Solna，Sweden）和E.根据美国国家临床实验室标准委员会（NCCLS）的指南，将粪肠球菌菌株分为敏感或耐药菌株。结果所有菌株在体外对青霉素敏感，但阿莫西林和阿莫西林-克拉维酸的MIC（MIC 90 = 0.75 mug mL（-1））低于苄青霉素（MIC 90 = 3.0 mug mL（-1））。所有研究的菌株也对万古霉素和氟沙星敏感，而95.2%的菌株对氯霉素敏感。对四环素和强力霉素敏感率为85.7%，对环丙沙星敏感率为80.9%。红霉素的MIC范围为0.38至>256 μ g mL（-1）;只有28.5%的菌株敏感（MIC小于或等于0.5 μ g mL（-1））。也观察到对阿奇霉素的有限敏感性，阿奇霉素仅对14.2%的分离株有效。结论粪肠球菌对阿莫西林、阿莫西林-克拉维酸、万古霉素和氟喹诺酮类抗生素完全敏感。大多数菌株对氯霉素、四环素、强力霉素和环丙沙星敏感。红霉素和阿奇霉素效果最差。
Aim To test, in vitro, the susceptibility to different antibiotics of Enterococcus faecalis isolates from canals of root filled teeth with periapical lesions.Methodology Twenty-one E. faecalis isolates, from canals of root filled teeth with persisting periapical lesions, were tested for their antibiotic susceptibilities. The following antibiotics were used: benzylpenicillin, amoxicillin, amoxicillin-clavulanic acid, erythromycin, azithromycin, vancomycin, chloramphenicol, tetracycline, doxycycline, ciprofloxacin and moxifloxacin. Minimal inhibitory concentrations (MICs) for the antimicrobial agents were determined using the E-test System (AB BIODISK, Solna, Sweden), and the E. faecalis strains classified as susceptible or resistant according to the guidelines of National Committee for Clinical Laboratory Standards (NCCLS). The strains were also tested for beta-lactamase production with nitrocefin (Oxoid, Basingstoke, UK).Results All strains were susceptible to penicillins in vitro, however, the MICs of amoxicillin and amoxicillin-clavulanic acid (MIC90 = 0.75 mug mL(-1)) were lower than for benzylpenicillin (MIC90 = 3.0 mug mL(-1)). All strains studied were also susceptible to vancomycin and moxifloxacin, whilst 95.2% were susceptible to chloramphenicol. Amongst the isolates, 85.7% were susceptible to tetracycline and doxycycline and 80.9% to ciprofloxacin. The MIC of erythromycin ranged from 0.38 to >256 mug mL(-1); only 28.5% of the strains were susceptible (MIC less than or equal to 0.5 mug mL(-1)). Limited susceptibility was also observed with azithromycin which was active against only 14.2% of isolates. No strains produced beta-lactamase.Conclusion Enterococcus faecalis isolates were completely susceptible, in vitro, to amoxicillin, amoxicillin-clavulanic acid, vancomycin and moxifloxacin. Most isolates were susceptible to chloramphenicol, tetracycline, doxycycline or ciprofloxacin. Erythromycin and azithromycin were least effective.