iTRAQ-based quantitative proteomics analysis of defense responses triggered by the pathogen Rhizoctonia solani infection in rice
iTRAQ-based quantitative proteomics analysis of defense responses triggered by the pathogen Rhizoctonia solani infection in rice
复制标题
基于 iTRAQ 的水稻病原菌立枯丝核菌感染引发的防御反应定量蛋白质组学分析
DOI：
10.1016/s2095-3119(20)63499-2
复制
发表时间：
2022-01-04
影响因子：
4.8
通讯作者：
Zuo Shi-min
中科院分区：
文献类型：
--
作者：
Feng Zhi-ming;Gao Peng;Zuo Shi-min
土传坏死性真菌立枯丝核菌(Rhizoctonia solani)是一种破坏性真菌，可导致多种重要作物严重减产。然而，人们对宿主针对这种病原体入侵的防御机制知之甚少。在本研究中，我们采用基于 iTRAQ 的定量蛋白质组学方法，利用抗性水稻品种 YSBR1 来研究对立枯丝核菌反应的宿主蛋白。总的来说，我们在用立枯丝核菌接种水稻植株后鉴定出了 319 种差异积累蛋白 (DAP)。功能分类分析表明这些 DAP 涵盖了广泛的功能。值得注意的是，大部分 DAP 参与细胞氧化还原稳态、碳水化合物代谢和苯丙素生物合成，或属于发病机制相关蛋白，表明这些过程/蛋白在宿主对抗立枯丝核菌的防御中发挥重要作用。有趣的是，所有参与光合作用和叶绿素生物合成过程的DAP，以及部分参与苯丙素生物合成的DAP，在茄丝菌感染后都显示出积累减少，这表明茄丝菌可能抑制宿主光合作用系统和苯丙素生物合成，以促进感染和定植。总之，我们的结果为水稻和立枯病菌相互作用的分子机制提供了宝贵的资源和新的见解。
The soil-borne necrotrophic fungus Rhizoctonia solani is one of destructive fungi causing severe yield losses in various important crops. However, the host defense mechanisms against the invasion of this pathogen are poorly understood. In this study, we employed an iTRAQ-based quantitative proteomic approach to investigate host proteins responsive to R. solani using the resistant rice cultivar YSBR1. As a whole, we identified 319 differentially accumulated proteins (DAPs) after inoculation of rice plants with R. solani. Functional categorization analysis indicates that these DAPs cover a broad range of functions. Notably, a substantial portion of the DAPs are involved in cell redox homeostasis, carbohydrate metabolism, and phenylpropanoid biosynthesis, or belong to pathogenesis-related proteins, indicating that these processes/proteins play important roles in host defense against R. solani. Interestingly, all of the DAPs involved in photosynthesis and chlorophyll biosynthetic processes, and part of the DAPs involved in phenylpropanoid biosynthesis, show reduced accumulation after R. solani infection, suggesting that R. solani probably inhibits host photosynthetic system and phenylpropanoid biosynthesis to facilitate infection and colonization. In conclusion, our results provide both valuable resources and new insights into the molecular mechanisms underlying rice and R. solani interaction.