Low glutelin content1:: A dominant mutation that suppresses the glutelin multigene family via RNA silencing in rice
Low glutelin content1:: A dominant mutation that suppresses the glutelin multigene family via RNA silencing in rice
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DOI：
10.1105/tpc.011452
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发表时间：
2003-06-01
期刊：
影响因子：
11.6
通讯作者：
Nishio, T
中科院分区：
文献类型：
--
作者：
Kusaba, M;Miyahara, K;Nishio, T
低谷蛋白含量1 （Lgc1）是水稻籽粒中降低谷蛋白含量的显性突变。谷朊蛋白是由多基因家族编码的主要种子贮藏蛋白。RNA凝胶印迹和逆转录介导的PCR分析显示，Lgc1在mRNA水平上以相似性依赖的方式起作用。在Lgc1纯合子中，两个高度相似的谷蛋白基因之间存在3.5 kb的缺失，形成尾对尾的反向重复，这可能产生双链RNA分子，这是一种有效的RNA沉默诱导剂。利用Lgc1候选区域进行转基因分析、报告基因分析和检测小干扰RNA，支持了Lgc1通过RNA沉默抑制谷蛋白表达的假设。在这种情况下，Lgc1提供了一个有趣的例子，说明在与RNA沉默诱导基因表现出不同程度相似性的基因之间发生RNA沉默。讨论了Lgc1介导谷蛋白多基因家族基因沉默的可能机制。
Low glutelin content1 (Lgc1) is a dominant mutation that reduces glutelin content in rice grains. Glutelin is a major seed storage protein encoded by a multigene family. RNA gel blot and reverse transcriptase-mediated PCR analyses revealed that Lgc1 acts at the mRNA level in a similarity-dependent manner. In Lgc1 homozygotes, there is a 3.5-kb deletion between two highly similar glutelin genes that forms a tail-to-tail inverted repeat, which might produce a double-stranded RNA molecule, a potent inducer of RNA silencing. The hypothesis that Lgc1 suppresses glutelin expression via RNA silencing is supported by transgenic analysis using this Lgc1 candidate region, by reporter gene analysis, and by the detection of small interfering RNAs. In this context, Lgc1 provides an interesting example of RNA silencing occurring among genes that exhibit various levels of similarity to an RNA-silencing-inducing gene. Possible mechanisms for gene silencing of the glutelin multigene family by Lgc1 are discussed.