Laboratory selection, cross-resistance, and estimations of realized heritability of indoxacarb resistance in Phenacoccus solenopsis (Homoptera: Pseudococcidae)
Laboratory selection, cross-resistance, and estimations of realized heritability of indoxacarb resistance in Phenacoccus solenopsis (Homoptera: Pseudococcidae)
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DOI：
10.1002/ps.5488
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发表时间：
2020-01-01
影响因子：
4.1
通讯作者：
Serrao, Jose E.
中科院分区：
文献类型：
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作者：
Afzal, Muhammad B. S.;Shad, Sarfraz A.;Serrao, Jose E.
背景：棉花粉蚧是棉花、蔬菜、蔬菜和药用植物的重要害虫。在世界许多地方，人们通过综合利用栽培、机械、生物和化学方法来管理茄属植物，但在巴基斯坦，杀虫剂喷雾剂的使用导致茄属植物对某些杀虫剂产生了抗药性。本研究通过在实验室条件下选择一个多世代的茄果类种群来研究其对茚虫威的抗性。结果：用茚虫威筛选27代后的田间种群对茚虫威的抗性水平为25623.17倍，抗性的遗传率为100%。茚虫威高抗种群对多杀菌素表现出很高的交互抗性，对联苯菊酯和毒死蜱表现出很低的交互抗性。对茚虫威的平均估计抗性h（2）仅为0.04。在h（2）= 0.04的条件下，对10%和95%的选择强度，预测产生10倍茚虫威抗性的世代数分别为346和36（斜率= 3.18）、237和25（斜率= 2.18）和128和13（斜率= 1.18）。然而，在斜率为1.18，选择强度为10%和95%的条件下，在128和13（h（2）= 0.04）、103和11（h（2）= 0.05）以及86和9（h（2）= 0.06）代后，茚虫威抗性也出现了同样的增加。本研究表明，室内筛选的结果表明，番茄对茚虫威具有很高的抗性。因此，在田间也可能出现对这种杀虫剂的抗性。由于茚虫威对联苯菊酯和/或毒死蜱的交叉抗性很低，可以通过将其与联苯菊酯和/或毒死蜱轮换使用，并避免与交叉抗性很高的多杀菌素轮换使用，来最大限度地减少茚虫威的抗性。(c)2019化学工业协会
BACKGROUND: Cotton mealybug Phenacoccus solenopsis is a pest of cotton, vegetables, ornamentals, and medicinal plants. In many parts of the world P. solenopsis has been managed by integration of cultural, mechanical, biological and chemical methods, but in Pakistan the use of insecticide sprays has resulted in the development of resistance to some insecticides. In this study indoxacarb resistance was investigated by selecting a P. solenopsis population under laboratory conditions for many generations. The cross-resistance potential of indoxacarb resistance with other chemistries and the realized heritability of indoxacarb resistance were also evaluated.RESULTS: A field population of P. solenopsis selected with indoxacarb for 27 generations had a 25 623.17-fold resistance level. The highly indoxacarb resistant population showed very high cross-resistance to spinosad and very low cross-resistance to bifenthrin and chlorpyrifos. The mean estimated h(2) of resistance to indoxacarb was only 0.04. The number of generations predicted for the development of ten-fold indoxacarb resistance at a constant h(2) = 0.04 against selection intensities of 10% and 95% were 346 and 36 (slope = 3.18), 237 and 25 (slope = 2.18) and 128 and 13 (slope = 1.18). However, at a constant slope = 1.18 and selection intensities of 10% and 95%, the same increase in indoxacarb resistance occurred after 128 and 13 (h(2) = 0.04), 103 and 11 (h(2) = 0.05), and 86 and 9 (h(2) = 0.06) generations.CONCLUSION: This study revealed that P. solenopsis has a very high resistance to indoxacarb as a result of laboratory selection. Thus, resistance to this insecticide in the field may also occur. Indoxacarb resistance can be minimized by rotating it with bifenthrin and/or chlorpyrifos due to its very low cross-resistance to these insecticides and by avoiding its rotation with Spinosad, which has very high cross-resistance. (c) 2019 Society of Chemical Industry