EVIDENCE FOR NEW TRANSCRIPTIONAL UNITS ENCODED AT THE 3' END OF THE MOUSE HEPATITIS-VIRUS GENOME
EVIDENCE FOR NEW TRANSCRIPTIONAL UNITS ENCODED AT THE 3' END OF THE MOUSE HEPATITIS-VIRUS GENOME
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DOI：
10.1006/viro.1993.1467
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发表时间：
1993-09-01
期刊：
影响因子：
3.7
通讯作者：
BARIC, RS
中科院分区：
文献类型：
--
作者：
SCHAAD, MC;BARIC, RS
Prior studies have demonstrated that the mouse hepatitis virus (MHV) genome is divided into at least seven coding regions each transcribed into a distinct mRNA. The majority of these mRNAs are synthesized from a highly conserved intergenic start site (UCU/CAAAC), contain a 65-72 nt leader RNA at their 5′ end and form a 3′ co-terminal nested set. In this study, we have used radiolabeling experiments to demonstrate the presence of a small ∼900 nt mRNA and its corresponding RF RNA in MHV-infected cells. Surprisingly, PCR amplification and sequence analysis revealed the presence of not one, but two small leader-containing RNAs that initiate from highly conserved intergenic start sites (UCCAAAC and UCUAAAU) which are located within the 3′-most nucleocapsid gene sequence. These studies provide evidence suggesting that one or two additional small mRNAs are encoded from the 3′ end of the MHV genome.
