Green tea extract attenuates MNU-induced photoreceptor cell apoptosis via suppression of heme oxygenase-1.
Green tea extract attenuates MNU-induced photoreceptor cell apoptosis via suppression of heme oxygenase-1.
复制标题
绿茶提取物通过抑制血红素加氧酶-1减轻了MNU诱导的感光细胞细胞凋亡。
DOI：
10.1293/tox.2015-0052
复制
发表时间：
2016-01
影响因子：
1.2
通讯作者：
Tsubura A
中科院分区：
文献类型：
--
作者：
Emoto Y;Yoshizawa K;Kinoshita Y;Yuki M;Yuri T;Tsubura A
研究了绿茶提取物（GTE）对n -甲基-n -亚硝基脲（MNU）诱导的光受体细胞凋亡的影响，并对其可能的作用机制进行了探讨。通过苏木精-伊红染色、vimentin免疫反应性和光感受器细胞凋亡（TUNEL标记）检测视网膜形态结构的改变。采用激光捕获显微解剖（LCM）法检测氧化标志物血红素加氧酶(HO)-1 mRNA表达水平。Sprague-Dawley大鼠在7周龄时给予40 mg/kg MNU，同时不给予250 mg/kg GTE（从MNU前3天开始每天1次，最多10天）。虽然光感受器细胞在MNU后24小时开始变性，但GTE在时间点的形态学影响并不确定。然而，GTE降低TUNEL标记和HO-1 mRNA的表达。在MNU后7天，GTE治疗减轻了光感受器损伤。因此，GTE减少mnu诱导的光感受器细胞凋亡的能力可能是由于其抗氧化特性。
The effects of green tea extract (GTE) on N-methyl-N-nitrosourea (MNU)-induced photoreceptor cell apoptosis were examined, and the possible mechanisms of action of GTE were assessed. Alterations in the retinal morphological architecture were determined by hematoxylin-eosin staining, vimentin immunoreactivity, and photoreceptor cell apoptosis (TUNEL labeling). Expression of oxidant marker, heme oxygenase (HO)-1, mRNA levels in outer nuclear cells was assessed by laser capture microdissection (LCM). Sprague-Dawley rats were given 40 mg/kg MNU at 7 weeks of age in the absence and presence of 250 mg/kg GTE treatment (once daily from 3 days prior to MNU for a maximum 10 days). Although photoreceptor cell degeneration began 24 hr after MNU, the morphological effects of GTE at the time point were not definitive. However, GTE lowered TUNEL labeling and HO-1 mRNA expression. At 7 days after MNU, photoreceptor damage was attenuated by GTE treatment. Therefore, the ability of GTE to reduce MNU-induced photoreceptor cell apoptosis may be due to its antioxidant properties.