Wild yeast harbour a variety of distinct amyloid structures with strong prion-inducing capabilities.
Wild yeast harbour a variety of distinct amyloid structures with strong prion-inducing capabilities.
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野生酵母具有多种独特的淀粉样蛋白结构，具有很强的朊病毒诱导能力。
DOI：
10.1111/mmi.12543
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发表时间：
2014
影响因子：
3.6
通讯作者：
True,HeatherL
中科院分区：
文献类型：
--
作者：
Westergard,Laura;True,HeatherL
淀粉样蛋白结构的变异深刻地影响了哺乳动物蛋白质构象紊乱的一系列病理表型，并且在酵母中主要是遗传表型。在这里，我们第一次描述了从酿酒酵母的野生菌株中分离出来的一种自然发生的、自我繁殖的、结构上的变异体。在各种野生酵母中繁殖的[RNQ+]Prion的变体在生化上不同，在细胞内的分布和促进[PSI+]Prion的形成方面也不同。[PSI+]是细胞表型和适应性的表观遗传调节因子。值得注意的是，我们发现大多数天然的[RNQ+]变体在高频下诱导[PSI+]，并且大多数[PSI+]变体引发强烈的细胞表型。我们假设，有效的[RNQ+]模板的存在为[PSI+]的形成启动了细胞，以便在有利的条件下诱导[PSI+]。这些研究利用自然产生的结构变异来扩大我们对不同的Pron构象对细胞表型的影响的理解。
Variation in amyloid structures profoundly influences a wide array of pathological phenotypes in mammalian protein conformation disorders and dominantly inherited phenotypes in yeast. Here, we describe, for the first time, naturally occurring, self‐propagating, structural variants of a prion protein isolated from wild strains of the yeastSaccharomyces cerevisiae. Variants of the [RNQ+] prion propagating in a variety of wild yeast differ biochemically, in their intracellular distributions, and in their ability to promote formation of the [PSI+] prion. [PSI+] is an epigenetic regulator of cellular phenotype and adaptability. Strikingly, we find that most natural [RNQ+] variants induced [PSI+] at high frequencies and the majority of [PSI+] variants elicited strong cellular phenotypes. We hypothesize that the presence of an efficient [RNQ+] template primes the cell for [PSI+] formation in order to induce [PSI+] in conditions where it would be advantageous. These studies utilize naturally occurring structural variants to expand our understanding of the consequences of diverse prion conformations on cellular phenotypes.