MicroRNA profiling of plasma exosomes from patients with ovarian cancer using high-throughput sequencing
MicroRNA profiling of plasma exosomes from patients with ovarian cancer using high-throughput sequencing
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DOI：
10.3892/ol.2019.10220
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发表时间：
2019-06-01
期刊：
影响因子：
2.9
通讯作者：
Liu, Xiaoli
中科院分区：
文献类型：
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作者：
Zhang, Honghong;Xu, Sijuan;Liu, Xiaoli
据我们所知，之前尚未研究过卵巢癌血浆外泌体的 microRNA (miR/miRNA) 表达谱。本研究的目的是探讨使用血浆外泌体 miRNA 作为卵巢癌新型血清学生物标志物的实用性。在这项研究中，利用差速离心从卵巢癌患者和健康女性的血浆中纯化出外泌体样囊泡。纯化的囊泡大小为 50-100 nm，通过透射电子显微镜和蛋白质印迹鉴定为外泌体。高通量测序表明，65 个已知 miRNA 在卵巢癌患者和健康女性之间存在差异表达，其中 34 个上调，31 个下调（P
To the best of our knowledge, the microRNA (miR/miRNA) expression profile of plasma exosomes in ovarian cancer has not been previously studied. The aim of the present study was to investigate the practicality of using plasma exosomal miRNAs as novel serological biomarkers of ovarian cancer. In the study, exosome-like vesicles were purified from the plasma of patients with ovarian cancer and healthy women using differential centrifugation. The purified vesicles, ranging from 50-100 nm in size, were identified as exosomes by transmission electron microscopy and western blotting. High-throughput sequencing demonstrated that 65 known miRNAs, 34 of which were upregulated and 31 downregulated, were differentially expressed between patients with ovarian cancer and healthy women (P