The PRP31 gene encodes a novel protein required for pre-mRNA splicing in Saccharomyces cerevisiae.
The PRP31 gene encodes a novel protein required for pre-mRNA splicing in Saccharomyces cerevisiae.
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DOI：
10.1093/nar/24.6.1164
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发表时间：
1996-03
影响因子：
14.9
通讯作者：
E. Weidenhammer;M. Singh;M. Ruiz-Noriega;J. Woolford
中科院分区：
文献类型：
--
作者：
E. Weidenhammer;M. Singh;M. Ruiz-Noriega;J. Woolford
The pre-mRNA splicing factor Prp31p was identified in a screen of temperature-sensitive yeast strains for those exhibiting a splicing defect upon shift to the non- permissive temperature. The wild-type PRP31 gene was cloned and shown to be essential for cell viability. The PRP31 gene is predicted to encode a 60 kDa polypeptide. No similarities with other known splicing factors or motifs indicative of protein-protein or RNA-protein interaction domains are discernible in the predicted amino acid sequence. A PRP31 allele bearing a triple repeat of the hemagglutinin epitope has been generated. The tagged protein is functional in vivo and a single polypeptide species of the predicted size was detected by Western analysis with proteins from yeast cell extracts. Functional Prp31p is required for the processing of pre-mRNA species both in vivo and in vitro, indicating that the protein is directly involved in the splicing pathway.
