Guanylate-binding protein 1 participates in cellular antiviral response to dengue virus.
Guanylate-binding protein 1 participates in cellular antiviral response to dengue virus.
复制标题
鸟苷酸结合蛋白1参与细胞对登革热病毒的抗病毒反应
DOI：
10.1186/1743-422x-9-292
复制
发表时间：
2012-11-27
期刊：
影响因子：
4.8
通讯作者：
Dai J
中科院分区：
文献类型：
--
作者：
Pan W;Zuo X;Feng T;Shi X;Dai J
背景登革病毒（DENV）是一种蚊媒病毒，广泛分布于世界各地的热带和亚热带地区，是引起人类登革出血热的病原体。目前还没有针对DENV的疫苗。鸟苷酸结合蛋白1（Guanylate binding protein 1，GBP 1）是干扰素（Interferon，IFN）刺激基因（Interferon stimulated gene，ISGs）之一，在宿主免疫防御中起重要作用。然而，GBP 1在DENV感染过程中的作用仍不清楚。本研究在体外模型中研究了GBP 1与登革病毒感染的相关性，结果发现，在登革病毒感染的RAW 264.7细胞中，小鼠Gbp 1的表达显著上调。Gbp 1基因沉默的细胞内DENV载量明显高于对照组。Gbp 1 siRNA处理的细胞中选择性抗病毒细胞因子的表达水平降低，而感染过程中，Gbp 1沉默导致NF-κB转录因子活性受损。
BackgroundDengue virus (DENV), the causative agent of human Dengue hemorrhagic fever, is a mosquito-borne virus found in tropical and sub-tropical regions around the world. Vaccines against DENV are currently unavailable. Guanylate-binding protein 1 (GBP1) is one of the Interferon (IFN) stimulated genes (ISGs) and has been shown important for host immune defense against various pathogens. However, the role of GBP1 during DENV infection remains unclarified. In this study, we evaluated the relevance of GBP1 to DENV infection inin vitromodel.FindingsQuantitative RT-PCR (qRT-PCR) and Western blot showed that the expression of mouseGbp1was dramatically upregulated in DENV-infected RAW264.7 cells. The intracellular DENV loads were significantly higher inGbp1silenced cells compared with controls. The expression levels of selective anti-viral cytokines were decreased inGbp1siRNA treated cells, while the transcription factor activity of NF-κB was impaired uponGBP1silencing during infection.ConclusionsOur data suggested that GBP1 plays an antiviral role during DENV infection.