Limitations on the spectrophotometric determination of copper(I) with ferrozine
Limitations on the spectrophotometric determination of copper(I) with ferrozine
复制标题
铁嗪分光光度法测定铜 (I) 的局限性
DOI：
10.1021/ac50025a040
复制
发表时间：
1978
影响因子：
7.4
通讯作者：
G. B. Ojo
中科院分区：
文献类型：
--
作者：
A. Anusiem;G. B. Ojo
先生：使用Ferrozine（每个化学公司），3-（2-吡啶基）-5，6-双（4-苯磺酸）-1，2，4-三嗪的二钠盐，用于测定铁、钴、铜、锇等金属离子，已有文献报道（7-3）。它还被用于间接测定还原三价铁的物质，如抗坏血酸（4），也用于测定液体样品中的二氧化硫（5）。1974年，Kundra等人。(2)结果表明，铜（I）和铁（II）可以同时测定含有两种离子的样品。他们表明，这种方法产生定量数据，并且不受其他离子的干扰。由于我们对血红蛋白和其他蛋白质的兴趣，我们的注意力被吸引到这种方法上。这似乎是一种有吸引力的方法，用于测定已知存在于几种生物大分子中的铜（6）。内格尔等(7)已经报道了铜与血红蛋白的结合，并表明这可能是研究蛋白质金属相互作用的良好模型。
Sir: The use of Ferrozine (Each Chemical Co.), thedisodium salt of 3 (2-pyridyl)-5, 6-bis (4-phenylsulfonic acid)-1, 2, 4-triazine, for the determination of several metallic ions such as iron, cobalt, copper, osmium, etc. has been reported by several workers (7-3). It has also been used for the indirect determination of species which reduce ferric iron such as ascorbic acid (4) and also for determining sulfur dioxide in liquid samples (5). In 1974, Kundra et al.(2) showed that copper (I) and iron (II) can be determined simultaneously in samples containing the two ions. They showed that this method yielded quantitative data and that it was free from interference by other ions. Our attention was drawn to this method because of our interest in hemoglobin and other proteins. This seemed to be an attractive method for the determination of copper which is known to be present in several biological macromolecules (6). Nagel et al.(7) have reported the binding of copper to hemoglobin andshowed that this could be a good model for the study of protein metal interaction.