A de novo originated gene depresses budding yeast mating pathway and is repressed by the protein encoded by its antisense strand
A de novo originated gene depresses budding yeast mating pathway and is repressed by the protein encoded by its antisense strand
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DOI：
10.1038/cr.2010.31
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发表时间：
2010-04-01
期刊：
影响因子：
44.1
通讯作者：
Wang, Wen
中科院分区：
文献类型：
--
作者：
Li, Dan;Dong, Yang;Wang, Wen
最近的转录图谱研究显示，在真核基因组中反义转录本的比例出人意料地高。这些反义基因似乎通过与正义基因相互作用来调节基因表达。以前的研究主要集中在非编码反义基因上，但对反义蛋白可能的调控作用知之甚少。在本研究中，我们发现由反义基因adf1编码的蛋白质作为转录抑制因子，调节正义基因mdf1在酿酒酵母中的表达。根据进化、遗传、细胞学和生化证据，我们认为编码蛋白质的正义基因MDF1很可能起源于之前的非编码序列，并通过结合MATα2显著抑制面包酵母在富含介质中的交配效率，从而促进营养生长。这些结果为几个重要问题提供了新的线索，包括一种新的正义-反义相互作用机制、功能基因的从头产生以及酵母交配途径的调控。
Recent transcription profiling studies have revealed an unexpectedly large proportion of antisense transcripts in eukaryotic genomes. These antisense genes seem to regulate gene expression by interacting with sense genes. Previous studies have focused on the non-coding antisense genes, but the possible regulatory role of the antisense protein is poorly understood. In this study, we found that a protein encoded by the antisense gene ADF1 acts as a transcription suppressor, regulating the expression of sense gene MDF1 in Saccharomyces cerevisiae. Based on the evolutionary, genetic, cytological and biochemical evidence, we show that the protein-coding sense gene MDF1 most likely originated de novo from a previously non-coding sequence and can significantly suppress the mating efficiency of baker's yeast in rich medium by binding MAT alpha 2 and thus promote vegetative growth. These results shed new light on several important issues, including a new sense-antisense interaction mechanism, the de novo origination of a functional gene, and the regulation of yeast mating pathway.