Static magnetic fields up-regulate osteoblast maturity by affecting local differentiation factors
Static magnetic fields up-regulate osteoblast maturity by affecting local differentiation factors
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DOI：
10.1097/01.blo.0000203464.35561.be
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发表时间：
2006-06-01
影响因子：
4.2
通讯作者：
Yeh, Ching-Ying
中科院分区：
文献类型：
--
作者：
Huang, Haw-Ming;Lee, Sheng-Yang;Yeh, Ching-Ying
细胞培养研究表明，静磁场在早期诱导成骨细胞分化。然而，分化效应的机制尚未得到很好的描述。我们假设静磁场通过调节细胞释放的早期局部因子刺激成骨细胞分化。为了验证我们的假设，MG63成骨细胞样细胞连续暴露于0.4 t静磁场中12、24、48和72小时。通过扫描电镜和透射电镜观察其形态变化和基质囊泡释放情况。比较静磁场对暴露和未暴露细胞转化生长因子- β 1、I型胶原蛋白、骨桥蛋白和碱性磷酸酶水平的影响。结果表明，静磁场处理的MG63细胞分化程度更高。静磁场处理细胞产生的局部调节因子大于对照细胞。这些发现为静磁场通过上调早期局部因子影响成骨细胞成熟提供了证据。
Cell culture studies have shown that static magnetic fields induce osteoblastic differentiation at an early stage. However, the mechanisms of differentiated effects have not been well described. We postulated that static magnetic fields stimulate osteoblastic differentiation by regulating early local factors released by the cells. To examine our hypothesis, MG63 osteoblast-like cells were exposed continuously to 0.4-T static magnetic fields for 12, 24, 48, and 72 hours. The morphologic changes and matrix vesicles release were observed by scanning and transmission electron microscopy. The effects of static magnetic fields on levels of transforming growth factor-beta 1, Type I collagen, osteopontin, and alkaline phosphatase were compared between the exposed and unexposed cells. The data suggest MG63 cells treated with static magnetic fields have more differentiated morphologic features. The local regulatory factors produced by static magnetic field-treated cells were greater than those of the control cultures. These findings provide evidence that static magnetic fields affect osteoblastic maturation by up-regulating early local factors.