Ethanol increases HSP70 concentrations in honeybee (Apis mellifera L.) brain tissue
Ethanol increases HSP70 concentrations in honeybee (Apis mellifera L.) brain tissue
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DOI：
10.1016/j.alcohol.2010.02.003
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发表时间：
2010-05-01
期刊：
影响因子：
2.3
通讯作者：
Carter, Richard P.
中科院分区：
文献类型：
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作者：
Hranitz, John M.;Abramson, Charles I.;Carter, Richard P.
以前对蜜蜂乙醇模型的研究确定了急性酒精暴露如何改变不同组织水平的功能：行为和学习、生态和生理。这项研究的目的是评估影响蜜蜂行为的乙醇剂量是否也会诱导蜜蜂脑组织中热休克蛋白70(HSP70)浓度的显著应激反应。实验1检测了三个预处理组的蜂脑HSP70浓度在采集后即刻、被套和喂食后以及套在套上22-24小时后对大脑HSP70浓度的影响。尽管我们观察到两个重复之间的HSP70浓度显著不同，但在重复内不同的预处理组之间HSP70浓度没有差异。实验2研究了乙醇剂量与脑HSP70浓度的关系。将蜜蜂分成7个试验组，其中3个预处理组与实验1相同，4个酒精饲料组。给酒精处理组的蜜蜂喂食1.5M蔗糖(对照组)和1.5M含2.5、5和10%乙醇的蔗糖-乙醇溶液，静置4h，并对大脑进行HSP70检测。我们观察到从对照组到5%的乙醇组，酒精诱导的蜜蜂脑中HSP70浓度的增加。只有5%乙醇组的蜜蜂HSP70浓度显著高于对照组。倒U型乙醇剂量-HSP70浓度反应曲线显示，摄入2.5%乙醇和5%乙醇可刺激应激反应，而摄入10%乙醇可抑制应激反应。显示HSP70最大浓度(5%乙醇)或HSP70抑制(10%乙醇)的剂量相当于先前研究中也损害蜜蜂的剂量(GT；=5%乙醇)。我们的结论是，含有5%乙醇的溶液急性乙醇中毒会在脑组织中引起显著的乙醇诱导的应激，从而损害蜜蜂的行为和联想学习。(C)2010 Elsevier Inc.保留所有权利。
Previous research on the honeybee ethanol model established how acute ethanol exposure altered function at different levels of organization: behavior and learning, ecology, and physiology. The purpose of this study was to evaluate whether ethanol doses that affect honeybee behavior also induce a significant stress response, measured by heat shock protein 70 (HSP70) concentrations, in honeybee brain tissues. Experiment 1 examined how pretreatment handling influenced brain HSP70 concentrations in three pretreatment groups of bees; immediately after being collected, after being harnessed and fed, and after 22-24 h in a harness. HSP70 concentrations did not differ among pretreatment groups within replicates, although we observed significantly different HSP70 concentrations between the two replicates. Experiment 2 investigated the relationship between ethanol dose and brain HSP70 concentrations. Bees were placed in seven experimental groups, the three pretreatment groups as in Experiment 1 and four ethanol-fed groups. Bees in ethanol treatments were fed 1.5 M sucrose (control) and 1.5 M sucrose-ethanol solutions containing 2.5, 5, and 10% ethanol, allowed to sit for 4 h, and dissected brains were assayed for HSP70. We observed ethanol-induced increases in honeybee brain HSP70 concentrations from the control group through the 5% ethanol group. Only bees in the 5% ethanol group had HSP70 concentrations significantly higher than the control group. The inverted U-shaped ethanol dose-HSP70 concentration response curve indicated that ingestion of 2.5% ethanol and 5% ethanol stimulated the stress response, whereas ingestion of 10% ethanol inhibited the stress response. Doses that show maximum HSP70 concentration (5% ethanol) or HSP70 inhibition (10% ethanol) correspond to those (>= 5% ethanol) that also impaired honeybees in previous studies. We conclude that acute ethanol intoxication by solutions containing >= 5% ethanol causes significant ethanol-induced stress in brain tissue that impairs honeybee behavior and associative learning. (C) 2010 Elsevier Inc. All rights reserved.