Genome analysis of an inducible prophage and prophage remnants integrated in the Streptococcus pyogenes strain SF370
Genome analysis of an inducible prophage and prophage remnants integrated in the Streptococcus pyogenes strain SF370
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DOI：
10.1006/viro.2002.1570
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发表时间：
2002-10-25
期刊：
影响因子：
3.7
通讯作者：
Brüssow, H
中科院分区：
文献类型：
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作者：
Canchaya, C;Desiere, F;Brüssow, H
从高致病性M1血清型化脓性链球菌SF370分离物中分离得到丝裂霉素C诱导的原噬菌体SF370.1，其基因组长41kb，其遗传组织与sf11样pac-site Siphoviridae相似。其最近的亲缘关系为M3血清型化脓性链球菌的NIH1.1噬菌体，其次是肺炎链球菌噬菌体MM1与乳酸菌噬菌体phig1e、李斯特菌噬菌体A118和噬菌体SPP1。与同一株多溶原菌株SF370的原噬菌体SF370.2和SF370.3序列的相似性主要局限于尾纤维基因。与其他两种噬菌体一样，SF370.1编码了噬菌体溶酶与正确附着位点之间可能的溶原转化基因。这些基因编码了化脓链球菌的热原外毒素C和一个与dna酶和有丝分裂因子序列相似的蛋白。SP370基因组的筛选进一步揭示了噬菌体样元件。全长13kb的噬菌体残体SF370.4编码溶原基因和DNA复制基因。在化脓链球菌（S. pyogenes）菌株Manfredo中，在相应的基因组位置发现了一个密切相关的前噬菌体残体。这两种噬菌体在内部索引和基因替换方面存在差异。检测到4种噬菌体样整合酶；其中三种仍然带有可能的抑制基因。所有的前噬菌体元件被整合到编码序列中。在所有病例中，噬菌体序列与编码序列互补。用噬菌体残体SF370.4分离DNA修复基因mutL和mutS；噬菌体SF370.1和肺炎链球菌噬菌体MM1整合到同源染色体位置。噬菌体序列的解释假设预测了噬菌体和宿主基因组之间的合作元素和军备竞赛。(C) 2002 Elsevier Science （USA）。
The mitomycin C inducible prophage SF370.1 from the highly pathogenic M1 serotype Streptococcus pyogenes isolate SF370 showed a 41-kb-long genome whose genetic organization resembled that of SF11-like pac-site Siphoviridae. Its closest relative was prophage NIH1.1 from an M3 serotype S. pyogenes strain, followed by S. pneumoniae phage MM1 and Lactobacillus phage phig1e, Listeria phage A118, and Bacillus phage SPP1 in a gradient of relatedness. Sequence similarity with the previously described prophages SF370.2 and SF370.3 from the same polylysogenic SF370 strain were mainly limited to the tail fiber genes. As in these two other prophages, SF370.1 encoded likely lysogenic conversion genes between the phage lysin and the right attachment site. The genes encoded the pyrogenic exotoxin C of S. pyogenes and a protein sharing sequence similarity with both DNases and mitogenic factors. The screening of the SP370 genome revealed further prophage-like elements. A 13-kb-long phage remnant SF370.4 encoded lysogeny and DNA replication genes. A closely related prophage remnant was identified in S. pyogenes strain Manfredo at a corresponding genome position. The two prophages differed by internal indels and gene replacements. Four phage-like integrases were detected; three were still accompanied by likely repressor genes. All prophage elements were integrated into coding sequences. The phage sequences complemented the coding sequences in all cases. The DNA repair genes mutL and mutS were separated by the prophage remnant SF370.4; prophage SF370.1 and S. pneumoniae phage MM1 integrated into homologous chromosomal locations. The prophage sequences were interpreted with a hypothesis that predicts elements of cooperation and an arms race between phage and host genomes. (C) 2002 Elsevier Science (USA).