Physical linkage of the constant region genes for immunoglobulins lambda I and lambda III.
Physical linkage of the constant region genes for immunoglobulins lambda I and lambda III.
复制标题
免疫球蛋白 lambda I 和 lambda III 恒定区基因的物理连接。
DOI：
10.1073/pnas.78.6.3829
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发表时间：
1981
影响因子：
11.1
通讯作者：
Storb,U
中科院分区：
文献类型：
--
作者：
Miller,J;Bothwell,A;Storb,U
在从干细胞向抗体分泌细胞分化的过程中，B细胞内的免疫球蛋白基因经历了一种重排，即可变区基因与恒定区基因并列。为了分析免疫球蛋白基因家族在未重排的生殖系DNA中的遗传组织，我们从随机切割的小鼠肾脏DNA片段中构建了重组DNA文库。从这个文库中，我们分离到了三个重叠的重组克隆，其中包含了lambda I轻链恒定区基因(C Lambda I)。这些克隆跨越24.9kb的小鼠DNA，不包含可变区序列。这些克隆与C lambda II基因的杂交结果表明，C lambda I存在一个额外的恒定区基因和一个3.2kb碱基的连接区，该基因被初步鉴定为C lambda III，因为在C lambda II中不存在Ava I酶切位点。娜塔莉。阿卡德。SCI。美国78,569-573]，与C lambda II氨基酸序列关系密切。
During differentiation from a stem cell to an antibody-secreting cell, the immunoglobulin genes within a B cell undergo a rearrangement that juxtaposes a variable region gene to a constant region gene. To analyze the genetic organization of an immunoglobulin gene family in nonrearranged, germ-line DNA, we have constructed a recombinant DNA library from randomly cleaved mouse kidney DNA fragments. From this library, we have isolated three overlapping recombinant clones containing the constant region gene for lambda I light chains (C lambda I). These clones spanned 24.9 kilobases of mouse DNA and contained no variable region sequences. Hybridization of these clones with lambda II cDNA demonstrated the presence of an additional constant region gene and a joining region 3.2 kilobases 5' of C lambda I. This gene was tentatively identified as C lambda III by the absence of an Ava I endonuclease site, which is present within C lambda II. The C lambda III amino acid sequence has recently been reported [Azuma, T., Steiner, L. A. & Eisen, H. N. (1981) Proc. Natl. Acad. Sci. USA 78, 569-573] and is very closely related to the C lambda II amino acid sequence.