Tumor-associated macrophages: The double-edged sword in cancer progression
Tumor-associated macrophages: The double-edged sword in cancer progression
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DOI：
10.1200/jco.2005.12.172
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发表时间：
2005-02-10
影响因子：
45.3
通讯作者：
Yang, PC
中科院分区：
文献类型：
--
作者：
Chen, JJW;Lin, YC;Yang, PC
炎症在癌症进展中起着至关重要的作用。在这项研究中，我们研究了肺癌细胞与巨噬细胞相互作用后的成瘤活性和基因表达谱。材料与方法我们测量了41例肺癌肿瘤标本的肿瘤内微血管计数和巨噬细胞密度，并将其与患者的临床预后联系起来。使用transwell共培养系统评估巨噬细胞与癌细胞系之间的相互作用。采用体外侵袭试验评价其侵袭潜能。采用明胶酶谱法测定基质降解活性。微阵列应用于参与相互作用的基因的大规模分析，以及监测共培养中抗炎药物反应的肺癌细胞的基因表达谱。结果巨噬细胞密度与微血管计数呈正相关，与患者无复发生存率呈负相关(P
Purpose Inflammation plays a critical role in cancer progression. In this study we investigate the protumorigenic activities and gene expression profiles of lung cancer cells after interaction with macrophages.Materials and Methods We measured intratumoral microvessel counts and macrophage density in 41 lung cancer tumor specimens and correlated these with the patients' clinical outcome. The interaction between macrophages and cancer cell lines was assessed using a transwell coculture system. The invasive potential was evaluated by in vitro invasion assay. The matrix-degrading activity was assayed by gelatin zymography. The microarray was applied to a large-scale analysis of the genes involved in the interaction, as well as to monitor the gene expression profiles of lung cancer cells responding to anti-inflammatory drugs in cocultures.Results The macrophage density positively correlated with microvessel counts and negatively correlated with patient relapse-free survival (P