PROBING THE STRUCTURE OF CYTOPLASM
PROBING THE STRUCTURE OF CYTOPLASM
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DOI：
10.1083/jcb.102.6.2015
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发表时间：
1986-06-01
影响因子：
7.8
通讯作者：
LANNI, F
中科院分区：
文献类型：
--
作者：
LUBYPHELPS, K;TAYLOR, DL;LANNI, F
我们已经使用了大小分级，荧光葡聚糖探针的结构的细胞质基质的生活瑞士3T3细胞通过荧光恢复后的光漂白和视频图像处理。这些数据表明，活细胞的细胞质具有比水大四倍的流体相粘度，并且含有以尺寸依赖性方式限制溶解的大分子自由扩散的结构屏障。假设这些结构屏障包括丝状网状物，光漂白后的组合荧光恢复和成像数据表明网状物的平均孔径在300至400埃的范围内，但是可以小到200埃。在一些细胞质区域。
We have used size-fractionated, fluorescent dextrans to probe the structure of the cytoplasmic ground substance of living Swiss 3T3 celsl by fluorescence recovery after photobleaching and video image processing. The data indicate that the cytoplasm of living cells has a fluid phase viscosity four times greater than water and contains structural barriers that restrict free diffusion of dissolved macromolecules in a size-dependent manner. Assuming these structural barriers comprise a filamentous meshwork, the combined fluorescence recovery after photobleaching and imaging data suggest that the average pore size of the meshwork is in the range of 300 to 400 .ANG., but may be as small as 200 .ANG. in some cytoplasmic domains.