Immunohistochemical demonstration of H2 antigens in mouse tissue sections.
Immunohistochemical demonstration of H2 antigens in mouse tissue sections.
复制标题
小鼠组织切片中 H2 抗原的免疫组织化学演示。
DOI：
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发表时间：
1983
影响因子：
3.2
通讯作者：
J. Westwood
中科院分区：
文献类型：
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作者：
B. Ponder;M. M. Wilkinson;M. Wood;J. Westwood
H2抗原在各种小鼠组织的冷冻切片的免疫组织学证明的报告。开发该方法的目的是使用H2抗原作为小鼠嵌合体研究中的细胞标志物。使用单克隆抗H2抗体，或者使用生物素或对氨基苯胂酸盐的半抗原夹心技术，或者作为直接酶缀合物。直接的抗体-酶结合物使用更简单，提供的特异性染色强度与用半抗原-夹心系统获得的强度相当，并且当尝试同时双重染色时，背景问题较少。这些结果提供了H2抗原在许多这些组织中的分布的描述。H2染色的强度在不同组织之间变化很大，但在组织内和同一窝小鼠之间也存在差异。放射自显影定量表明，有一个可用的H2抗原的五倍的变化之间的组织染色强或弱，我们的技术。
The immunohistological demonstration of H2 antigens in cryostat sections of a wide variety of mouse tissues is reported. The purpose in developing the method was to use H2 antigens as cellular markers in studies of mouse chimeras. Monoclonal anti-H2 antibodies were used, either with a hapten-sandwich technique using biotin or arsanilate, or as direct enzyme conjugates. The direct antibody-enzyme conjugates were simpler to use, provided an intensity of specific staining which was comparable to that obtained with the hapten-sandwich systems, and gave fewer problems of background when simultaneous double staining was attempted. The results provide a description of the distribution of H2 antigens in many of these tissues. The intensity of H2 staining varied widely from tissue to tissue, but also within tissues and between individual mice of the same litter. Quantitation by autoradiography suggests that there is a fivefold variation in available H2 antigen between tissues which are stained strongly or weakly by our technique.