Definitive evidence for the acute sarin poisoning diagnosis in the Tokyo subway.
Definitive evidence for the acute sarin poisoning diagnosis in the Tokyo subway.
复制标题
DOI：
10.1006/taap.1997.8110
复制
发表时间：
1997-05
影响因子：
3.8
通讯作者：
Masataka Nagao;T. Takatori;Yukimasa Matsuda;Makoto Nakajima;Hirotaro Iwase;Kimiharu Iwadate
中科院分区：
文献类型：
--
作者：
Masataka Nagao;T. Takatori;Yukimasa Matsuda;Makoto Nakajima;Hirotaro Iwase;Kimiharu Iwadate
开发了一种新方法，用于检测东京地铁恐怖袭击造成的四名沙林（甲基膦氟酸异丙酯）中毒受害者的红细胞中的沙林水解产物。从沙林受害者红细胞膜中溶解出沙林结合乙酰胆碱酯酶（AChE），用胰蛋白酶消化，碱性磷酸酶消化释放出与AChE结合的沙林水解产物，将消化的沙林水解产物进行三甲基甲硅烷基衍生化，并通过气相色谱-质谱联用法检测。在我们检查的所有沙林中毒受害者中都检测到了异丙基甲基膦酸（一种沙林水解产物），并且在所有受害者中都检测到了甲基膦酸（一种沙林和索曼水解产物）。尸检未发现沙林中毒特有的宏观和微观发现，并且沙林及其水解产物在他们的血液中几乎检测不到。我们认为下面描述的程序将有助于急性沙林中毒的法医诊断。
A new method was developed to detect sarin hydrolysis products from erythrocytes of four victims of sarin (isopropylmethylphosphonofluoridate) poisoning resulting from the terrorist attack on the Tokyo subway. Sarin-bound acetylcholinesterase (AChE) was solubilized from erythrocyte membranes of sarin victims, digested with trypsin, the sarin hydrolysis products bound to AChE were released by alkaline phosphatase digestion, and the digested sarin hydrolysis products were subjected to trimethylsilyl derivatization and detected by gas chromatography-mass spectrometry. Isopropylmethylphosphonic acid, which is a sarin hydrolysis product, was detected in all sarin poisoning, victims we examined and methylphosphonic acid, which is a sarin and soman hydrolysis product, was determined in all victims. Postmortem examinations revealed no macroscopic and microscopic findings specific to sarin poisoning and sarin and its hydrolysis products were almost undetectable in their blood. We think that the procedure described below will be useful for the forensic diagnosis of acute sarin poisoning.