Comparison of approaches to the instrumental response function in fluorescence decay measurements.
Comparison of approaches to the instrumental response function in fluorescence decay measurements.
复制标题
荧光衰减测量中仪器响应函数方法的比较。
DOI：
10.1016/0003-2697(86)90111-9
复制
发表时间：
1986
影响因子：
2.9
通讯作者：
Barkley,MD
中科院分区：
文献类型：
--
作者：
Kolber,ZS;Barkley,MD
脉冲荧光测定数据的去卷积需要仪器响应的知识，这在某些情况下是不能直接观察到的。纳秒荧光衰减数据分析的非线性最小二乘法，包括常用的时移校正和几个参考荧光团的方法进行了评估，接近仪器响应函数的各种程序。一种新的参考荧光团技术，使用Monte Carlo卷积的介绍和测试。还介绍了几种参考技术中散射光的校正。随机卷积和另一种参考荧光团方法在广泛的实验条件下一致地给出了上级结果。
Deconvolution of pulse fluorometry data requires knowledge of the instrumental response, which is not directly observable in some circumstances. Various procedures for approaching the instrumental response function were evaluated for nanosecond fluorescence decay data analyzed by nonlinear least squares, including the commonly used time shift correction and several reference fluorophore methods. A new reference fluorophore technique using a Monte Carlo convolution is introduced and tested. The correction for scattered light in several reference techniques is also presented. The random convolution and one other reference fluorophore method consistently gave superior results over a wide range of experimental conditions.