Application of 13C isotope labeling using liquid chromatography mass spectrometry (LC-MS) to determining phosphate-containing metabolic incorporation.
Application of 13C isotope labeling using liquid chromatography mass spectrometry (LC-MS) to determining phosphate-containing metabolic incorporation.
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DOI：
10.1002/jms.3292
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发表时间：
2013
期刊：
影响因子：
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通讯作者：
S. K. Bhowmik;V. Putluri;R. Kommagani;S. A. Konde;J. Lydon;A. Sreekumar;N. Putluri
中科院分区：
文献类型：
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作者：
S. K. Bhowmik;V. Putluri;R. Kommagani;S. A. Konde;J. Lydon;A. Sreekumar;N. Putluri
在这里，我们描述了一种方法，其中负电喷雾电离质谱已用于了解通过磷酸盐的代谢中间体与中央碳代谢后，管理细胞与13 C-标记的基板相关的相对通量。该方法被应用于检查T47 D乳腺癌细胞中通过糖酵解的13 C掺入，并显示在用2-脱氧葡萄糖处理后糖酵解相对通量的降低。
Here, we describe an approach wherein negative electrospray ionization mass spectrometry has used to understand the relative flux through phosphate containing metabolic intermediates associated with central carbon metabolism after administering cells with 13C-labeled substrates. The method was applied to examine the 13C incorporation through glycolysis in T47D breast cancer cells and showed reduction of glycolytic relative flux upon treatment with 2-Deoxyglucose.