Effects of 2,3-Dimercaptosuccinic Acid-Coated Fe3O4 Nanoparticles on Genes in Two Mouse Cell Lines
Effects of 2,3-Dimercaptosuccinic Acid-Coated Fe3O4 Nanoparticles on Genes in Two Mouse Cell Lines
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2,3-二巯基丁二酸包覆的 Fe3O4 纳米粒子对两种小鼠细胞系基因的影响
DOI：
10.1166/jbn.2014.1843
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发表时间：
2014-08-01
影响因子：
2.9
通讯作者：
Wang, Jinke
中科院分区：
文献类型：
--
作者：
Liu, Yingxun;Zou, Jinglu;Wang, Jinke
为了评估氧化铁纳米颗粒对不同细胞系基因的影响，本研究采用dmsa包被的Fe3O4纳米颗粒处理小鼠巨噬细胞RAW264.7和肝细胞Hepa1-6细胞系。分别使用50 μ g/mL和100 μ g/mL两种剂量，作用24小时，通过DNA微阵列检测基因表达谱。两种细胞系的基因表达模式在比较时差异很大，揭示了纳米颗粒对基因的不同细胞特异性作用。结果发现，纳米颗粒对RAW264.7细胞中基因的表达有显著影响，与Hepa1-6细胞完全不同。两种剂量的纳米颗粒在RAW264.7细胞中下调了更多的基因，而在Hepa1-6细胞中上调了更多的基因。此外，纳米颗粒剂量的增加大大降低了RAW264.7细胞中基因的上调，但增加了基因的下调。纳米颗粒的影响在Hepa1-6细胞中没有产生类似的效果。除了两种细胞系基因表达模式的差异外，两种细胞系中分别有8个和7个基因在低剂量和高剂量纳米颗粒作用下持续下调和上调，揭示了纳米颗粒对两种细胞系基因的共同作用。这些对基因的共同影响是由稳态过程引起的，受基因下调和免疫反应的影响，也受基因上调影响的细胞死亡。本研究的数据为dmsa包覆的Fe3O4纳米颗粒的潜在体内纳米毒理学提供了新的见解。
To evaluate the effects of iron oxide nanoparticles on genes from different cell lines in this study, mouse macrophage RAW264.7 and hepatocyte Hepa1-6 cell lines were treated with DMSA-coated Fe3O4 nanoparticles. Two doses were used, 50 mu g/mL and 100 mu g/mL, respectively, for 24 hr and gene expression profile was detected by DNA microarrays. The gene expression patterns for the two cell lines greatly differed from each other when compared, revealing distinct cell-specific effects of the nanoparticles on the genes. It was found that the nanoparticles significantly influenced expression of genes in the RAW264.7 cells, showing complete difference from those of Hepa1-6 cells. More genes were down-regulated in the RAW264.7 cells by two doses of the nanoparticles but up-regulated in the Hepa1-6 cells by two doses of the same nanoparticles. Moreover, the increase of nanoparticle dose greatly decreased up-regulation of genes but increased down-regulation of genes in the RAW264.7 cells. The influence of nanoparticles did not result in similar effect in the Hepa1-6 cells. Apart from the difference in gene expression patterns in the two cell lines, there were eight and seven genes which were consistently down-regulated and up-regulated by low-dose and high-dose of nanoparticles in the two cell lines, respectively, revealing the common effects of the nanoparticles on the genes in the two cell lines. These common effects on genes were caused by homeostatic processes, influenced by down-regulation of genes and immune responses, and also cell death, influenced by up-regulation of genes. The data from this study has shed new insights into the potential in vivo nanotoxicolgy of the DMSA-coated Fe3O4 nanoparticles.