Simple disposable microfluidic device for Salmonella typhimurium detection by magneto-immunoassay
Simple disposable microfluidic device for Salmonella typhimurium detection by magneto-immunoassay
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DOI：
10.1016/j.snb.2017.08.075
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发表时间：
2018-02-01
影响因子：
8.4
通讯作者：
Faria, Ronaldo Censi
中科院分区：
文献类型：
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作者：
de Oliveira, Tassia Regina;Martucci, Diego Henrique;Faria, Ronaldo Censi
在此，我们描述了一种简单、低成本、完全一次性的微流体装置（D mu FD）的构建，用于超灵敏的鼠伤寒沙门氏菌（S.typhi）检测。D mu FD基于未修饰的碳电极阵列，以金纳米颗粒（AuNPs）为标记，通过磁免疫分析法快速检测牛奶样品中的伤寒沙门氏菌。该方法先用单克隆抗沙门菌抗体修饰磁珠，再用AuNPs标记多克隆抗沙门菌抗体，对样品进行磁捕获和分离。将形成的磁免疫偶联物注射到D mu FD中，并通过在未修饰的工作电极后面放置磁铁在电极表面捕获。利用AuNPs中金的电化学响应进行伤寒沙门氏菌的检测。采用单D μ FD，可同时测定标准品和样品，快速、全面地分析伤寒沙门氏菌。在10.0 ~ 100.0 cells mL（-1）的线性范围内，伤寒沙门氏菌的检出下限为7.7 cells mL（-1）。结果表明，该方法快速、准确、廉价，在多种食品污染物的多重检测中具有很大的潜力。(C) 2017 Elsevier B.V.版权所有
Herein, we describe the construction of a simple, low-cost, and fully disposable microfluidic device (D mu FD) for ultrasensitive Salmonella typhimurium (S.typhi) detection. The D mu FD is based on unmodified carbon electrode array for rapid detection of S. typhi in milk samples by magneto-immunoassay using gold nanoparticles (AuNPs) as a label. In the method proposed, S. typhi was magnetically captured and separated from samples using magnetic beads modified with a monoclonal anti-Salmonella antibody followed by the addition of polyclonal anti-Salmonella antibody labeling with AuNPs. The magneto-immunoconjugate formed was injected into the D mu FD and captured on the electrode surface by placing magnets behind the unmodified working electrodes. The S. typhi detection was carried out by the electrochemical response of the gold from AuNPs. A single D mu FD was used for rapid and full S. typhi analysis by measuring standards and samples simultaneously. A low limit of detection (LOD) of 7.7 cells mL(-1) for S. typhi in a linear range from 10.0 to 100.0 cells mL(-1) was obtained. The D FD proved to be fast, accurate, and inexpensive for S. typhi detection and has great potential to multiplex detection of different food contaminants. (C) 2017 Elsevier B.V. All rights reserved.