Salt Concentration Effects on Equilibrium Melting Curves from DNA Microarrays
Salt Concentration Effects on Equilibrium Melting Curves from DNA Microarrays
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DOI：
10.1016/j.bpj.2010.07.002
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发表时间：
2010-09-22
影响因子：
3.4
通讯作者：
Livache, T.
中科院分区：
文献类型：
--
作者：
Fuchs, J.;Fiche, J. -B.;Livache, T.
DNA microarrays find applications in an increasing number of domains where more quantitative results are required. DNA being a charged polymer, the repulsive interactions between the surface of the microarray and the targets in solution are increasing upon hybridization. Such electrostatic penalty is generally reduced by increasing the salt concentration. In this article, we present equilibrium-melting curves obtained from dedicated physicochemical experiments on DNA microarrays in order to get a better understanding of the electrostatic penalty incurred during the hybridization reaction at the surface. Various salt concentrations have been considered and deviations from the commonly used Langmuir adsorption model are experimentally quantified for the first time in agreement with theoretical predictions.
