Novel Hantavirus in the Flat-Skulled Shrew (Sorex roboratus)
Novel Hantavirus in the Flat-Skulled Shrew (Sorex roboratus)
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DOI：
10.1089/vbz.2009.0159
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发表时间：
2010-08-01
影响因子：
2.1
通讯作者：
Yanagihara, Richard
中科院分区：
文献类型：
--
作者：
Kang, Hae Ji;Arai, Satoru;Yanagihara, Richard
最近在欧亚大陆和北美的多种鼩鼱（鼩目，鼩科）中发现了遗传上独特的汉坦病毒。为了证实几十年前在俄罗斯鼩鼱中发现汉坦病毒抗原的报道，2006年7月和8月在西伯利亚东北部萨哈共和国捕获的4只西伯利亚大齿鼩鼱（Sorex daphaenodon）、5只欧亚最小齿鼩鼱（Sorex minutissimus）、12只平颅鼩鼱（Sorex roboratus）和18只苔原鼩鼱（Sorex tundrensis）的肝脏和肺组织档案采用逆转录-聚合酶链反应分析了汉坦病毒RNA。一种名为肯肯病毒的新型汉坦病毒在一只平颅鼩鼱身上被发现。全长S和部分M和L片段的序列分析表明，Kenkeme病毒在遗传和系统发育上不同于欧亚普通鼩鼱（Sorex araneus）携带的Seewis病毒，以及所有其他啮齿动物、固体动物和蟾蜍携带的汉坦病毒。
Genetically distinct hantaviruses have been identified recently in multiple species of shrews (Order Soricomorpha, Family Soricidae) in Eurasia and North America. To corroborate decades-old reports of hantaviral antigens in shrews from Russia, archival liver and lung tissues from 4 Siberian large-toothed shrews (Sorex daphaenodon), 5 Eurasian least shrews (Sorex minutissimus), 12 flat-skulled shrews (Sorex roboratus), and 18 tundra shrews (Sorex tundrensis), captured in the Sakha Republic in northeastern Siberia during July and August 2006, were analyzed for hantavirus RNA by reverse transcription-polymerase chain reaction. A novel hantavirus, named Kenkeme virus, was detected in a flat-skulled shrew. Sequence analysis of the full-length S and partial M and L segments indicated that Kenkeme virus was genetically and phylogenetically distinct from Seewis virus harbored by the Eurasian common shrew (Sorex araneus), as well as all other rodent-, soricid-, and talpid-borne hantaviruses.