Arabidopsis DREB1B in transgenic Salvia miltiorrhiza increased tolerance to drought stress without stunting growth
Arabidopsis DREB1B in transgenic Salvia miltiorrhiza increased tolerance to drought stress without stunting growth
复制标题
转基因丹参中的拟南芥 DREB1B 提高了对干旱胁迫的耐受性而不阻碍生长
DOI：
10.1016/j.plaphy.2016.03.003
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发表时间：
2016-07-01
影响因子：
6.5
通讯作者：
Zhang, Yong
中科院分区：
文献类型：
--
作者：
Wei, Tao;Deng, Kejun;Zhang, Yong
多个逆境反应基因受转录因子的协同调控，因此这些因子可用于分子植物育种。已知的逆境诱导基因CBF1/DREB1B是从拟南芥中分离得到的，并在CaMV35S或RD29A启动子的控制下导入丹参。干旱胁迫下，转基因植株的相对含水量、叶绿素含量和净光合速率均高于野生型植株。此外，在转基因品系中观察到O-2(-)和H_2O_2积累较低。进一步的分析表明，在干旱胁迫下，转AtDREB1B基因植株的丙二醛(MDA)含量低于对照植株，而超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和过氧化物酶(POD)活性高于对照植株。对参与光合作用、逆境反应、碳水化合物代谢和细胞保护的一组基因进行实时定量聚合酶链式反应，进一步证实了AtDREB1B通过激活不同的下游DREB/CBF基因来增强转基因植株的抗旱性。此外，在CaMV35S启动子或胁迫诱导的RD29A启动子驱动下表达AtDREB1B的转基因丹参植株中没有检测到生长抑制。综上所述，这些结果表明AtDREB1B是提高转基因丹参耐旱性的一个很好的候选基因。(C)2016年爱思唯尔·马森SAS。版权所有。
Multiple stress response genes are controlled by transcription factors in a coordinated manner; therefore, these factors can be used for molecular plant breeding. CBF1/DREB1B, a known stress-inducible gene, was isolated from Arabidopsis thaliana and introduced into Salvia miltiorrhiza under the control of the CaMV35S or RD29A promoter. Under drought stress, relative water content, chlorophyll content, and the net photosynthetic rate were observed to be higher in the transgenic lines than in the wild type (WT). Moreover, O-2(-) and H2O2 accumulation was observed to be lower in the transgenic lines. Additional analyses revealed that the AtDREB1B transgenic plants generally displayed lesser malondialdehyde (MDA) but higher superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) activities than the WT under drought stress. Quantitative real-time polymerase chain reaction of a subset of genes involved in photosynthesis, stress response, carbohydrate metabolism, and cell protection further verified that AtDREB1B could enhance tolerance to drought by activating different downstream DREB/CBF genes in the transgenic plants. Furthermore, no growth inhibition was detected in transgenic S. miltiorrhiza plants that expressed AtDREB1B driven by either the constitutive CaMV35S promoter or the stress-inducible RD29A promoter. Together, these results suggest that AtDREB1B is a good candidate gene for increasing drought tolerance in transgenic S. miltiorrhiza. (C) 2016 Elsevier Masson SAS. All rights reserved.