The Novel Gene VpPR4-1 from Vitis pseudoreticulata Increases Powdery Mildew Resistance in Transgenic Vitis vinifera L.
The Novel Gene VpPR4-1 from Vitis pseudoreticulata Increases Powdery Mildew Resistance in Transgenic Vitis vinifera L.
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DOI：
10.3389/fpls.2016.00695
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发表时间：
2016
影响因子：
5.6
通讯作者：
Zhang J
中科院分区：
文献类型：
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作者：
Dai L;Wang D;Xie X;Zhang C;Wang X;Xu Y;Wang Y;Zhang J
病程相关蛋白(PR)可以提高植物对病原菌侵袭的抗性。在这里，我们从野生中国葡萄中分离并鉴定了一个PR-4蛋白(VpPR4-1)，该蛋白在白粉病侵染后表现出显著的转录上调。我们还研究了它在各种非生物胁迫下的表达谱。白粉菌、水杨酸和茉莉酸甲酯显著增加了VpPR4-1的表达，而氯化钠和热处理仅轻微诱导了VpPR4-1的表达。脱落酸和冷处理对VpPR4-1的表达水平影响不大。VpPR4-1基因在30株再生葡萄中过表达。通过根癌农杆菌介导法转化红地球，并经Western印迹鉴定。26株转基因葡萄的PR-4蛋白含量表达水平高于野生型葡萄，其中6株接种了白粉病，表明白粉病的生长受到抑制。接种白粉病可提高转VpPR4-1基因红地球的抗白粉病能力。此外，由于VpPR4-1基因丰富，其他白粉病抗性基因也与反馈调控相关。这项研究表明，葡萄中的PR-4蛋白在抵御白粉病侵袭中起着至关重要的作用。
Pathogenesis-related proteins (PRs) can lead to increased resistance of the whole plant to pathogen attack. Here, we isolate and characterize a PR-4 protein (VpPR4-1) from a wild Chinese grape Vitis pseudoreticulata which shows greatly elevated transcription following powdery mildew infection. Its expression profiles under a number of abiotic stresses were also investigated. Powdery mildew, salicylic acid, and jasmonic acid methyl ester significantly increased the VpPR4-1 induction while NaCl and heat treatments just slightly induced VpPR4-1 expression. Abscisic acid and cold treatment slightly affected the expression level of VpPR4-1. The VpPR4-1 gene was overexpressed in 30 regenerated V. vinifera cv. Red Globe via Agrobacterium tumefaciens-mediated transformation and verified by the Western blot. The 26 transgenic grapevines exhibited higher expression levels of PR-4 protein content than wild-type vines and six of them were inoculated with powdery mildew which showed that the growth of powdery mildew was repressed. The powdery mildew-resistance of Red Globe transformed with VpPR4-1 was enhanced inoculated with powdery mildew. Moreover, other powdery mildew resistant genes were associated with feedback regulation since VpPR4-1 is in abundance. This study demonstrates that PR-4 protein in grapes plays a vital role in defense against powdery mildew invasion.