Identification of myogenic regulatory genes in the muscle transcriptome of beltfish (Trichiurus lepturus): A major commercial marine fish species with robust swimming ability.
Identification of myogenic regulatory genes in the muscle transcriptome of beltfish (Trichiurus lepturus): A major commercial marine fish species with robust swimming ability.
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带鱼（Trichiurus lepturus）肌肉转录组中生肌调节基因的鉴定：具有强大游泳能力的主要商业海洋鱼类
DOI：
10.1016/j.gdata.2016.04.005
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发表时间：
2016-06
期刊：
影响因子：
--
通讯作者：
Hsiao CD
中科院分区：
文献类型：
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作者：
Zhang H;Chang CM;Shen KN;Xian W;Hsiao CD
带鱼（Trichiurus lepturus）被认为是东亚最重要的经济海鱼之一。它是一种具有强大游泳能力的顶级捕食者，是研究鱼类肌肉生理的良好模型。本研究采用Illumina测序技术（NextSeq500）对带鱼幼鱼肌肉转录组进行测序、组装和注释。RNA测序共获得57,509,280个clean reads（存储在NCBI SRA数据库中，登录号为SRX1674471），使用Trinity软件重新组装后获得26,811个unigenes （N50为1033 bp）。BLASTX对NR、GO、KEGG和eggNOG数据库的标注率分别为100%、49%、31%和96%。通过对带鱼肌肉转录组的挖掘，鉴定出几个在肌肉发生调控中起重要作用的关键基因，包括pax3、pax7、myf5、myoD、mrf4/myf6、myogenin和myostatin，它们的表达水平较低。条带鱼的肌肉转录组可以为硬骨鱼肌肉组织全基因组转录组谱的研究提供一些见解，并为幼鱼/成鱼肌肉发生的研究提供有用的信息。
The beltfish (Trichiurus lepturus) is considered as one of the most economically important marine fish in East Asia. It is a top predator with a robust swimming ability that is a good model to study muscle physiology in fish. In the present study, we used Illumina sequencing technology (NextSeq500) to sequence, assemble and annotate the muscle transcriptome of juvenile beltfish. A total of 57,509,280 clean reads (deposited in NCBI SRA database with accession number of SRX1674471) were obtained from RNA sequencing and 26,811 unigenes (with N50 of 1033 bp) were obtained after de novo assembling with Trinity software. BLASTX against NR, GO, KEGG and eggNOG databases show 100%, 49%, 31% and 96% annotation rate, respectively. By mining beltfish muscle transcriptome, several key genes which play essential role on regulating myogenesis, including pax3, pax7, myf5, myoD, mrf4/myf6, myogenin and myostatin were identified with a low expression level. The muscle transcriptome of beltfish can provide some insight into the understanding of genome-wide transcriptome profile of teleost muscle tissue and give useful information to study myogenesis in juvenile/adult fish.