Fimbria-fornix transections selectively down-regulate subtypes of glutamate transporter and glutamate receptor proteins in septum and hippocampus.
Fimbria-fornix transections selectively down-regulate subtypes of glutamate transporter and glutamate receptor proteins in septum and hippocampus.
复制标题
穹窿伞横断选择性下调隔膜和海马中谷氨酸转运蛋白和谷氨酸受体蛋白的亚型。
DOI：
10.1046/j.1471-4159.1996.67031208.x
复制
发表时间：
1996
影响因子：
4.7
通讯作者：
Martin,LJ
中科院分区：
文献类型：
--
作者：
Ginsberg,SD;Rothstein,JD;Price,DL;Martin,LJ
在单侧穹窿海马伞横断后的成年大鼠中评估中枢神经系统轴切术对谷氨酸转运蛋白和谷氨酸受体表达的影响。在病变后第3、7、14和30天收集隔膜和海马。使用针对谷氨酸转运蛋白（GLT-1、GLAST 和 EAAC1）和谷氨酸受体（GluR1、GluR2/3、GluR6/7 和 NMDAR1）的抗体对匀浆进行免疫印迹，并通过d-[3H]天冬氨酸结合来检测谷氨酸转运。病变后 7 天和 14 天，同侧隔膜内和海马内 GLT-1 减少。损伤后 7 天和 14 天，同侧隔膜和海马内 GLAST 下降。没有观察到 EAAC1 免疫反应性的损伤后改变。d-[3H]天冬氨酸结合在同侧隔膜内和损伤后 14 天海马内于损伤后 7、14 和 30 天减少。病变后 30 天，同侧隔膜内的 GluR2/3 表达下调，而 GluR1、GluR6/7 和 NMDAR1 免疫反应性未改变。此外，在海马匀浆中未检测到谷氨酸受体表达的变化。这项研究表明，中枢神经系统内区域传入神经阻滞后，主要是胶质细胞而非神经元谷氨酸转运蛋白的选择性下调，以及间隔 GluR2/3 受体表达的延迟、亚型特异性下调。
The effects of CNS axotomy on glutamate transporter and glutamate receptor expression were evaluated in adult rats following unilateral fimbria‐fornix transections. The septum and hippocampus were collected at 3, 7, 14, and 30 days postlesion. Homogenates were immunoblotted by using antibodies directed against glutamate transporters (GLT‐1, GLAST, and EAAC1) and glutamate receptors (GluR1, GluR2/3, GluR6/7, and NMDAR1), and they were assayed for glutamate transport byd‐[3H]aspartate binding. GLT‐1 was decreased at 7 and 14 days postlesion within the ipsilateral septum and at 7 days postlesion in the hippocampus. GLAST was decreased within the ipsilateral septum and hippocampus at 7 and 14 days postlesion. No postlesion alterations in EAAC1 immunoreactivity were observed.d‐[3H]Aspartate binding was decreased at 7, 14, and 30 days postlesion within the ipsilateral septum and 14 days postlesion in the hippocampus. GluR2/3 expression was down‐regulated at 30 days postlesion within the ipsilateral septum, whereas GluR1, GluR6/7, and NMDAR1 immunoreactivity was unchanged. In addition, no alterations in glutamate receptor expression were detected within hippocampal homogenates. This study demonstrates a selective down‐regulation of primarily glial, and not neuronal, glutamate transporters and a delayed, subtype‐specific down‐regulation of septal GluR2/3 receptor expression after regional deafferentation within the CNS.