Measurement of bisphenol A levels in human urine
Measurement of bisphenol A levels in human urine
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DOI：
10.1038/sj.jea.7500174
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发表时间：
2001-07-01
期刊：
影响因子：
--
通讯作者：
Needham, LL
中科院分区：
文献类型：
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作者：
Brock, JW;Yoshimura, Y;Needham, LL
我们报告了一种新的方法来评估人类暴露于双酚a （BPA）通过测量尿液中的双酚a酶脱糖醛酸处理后。该方法包括添加（13）C（12）标记的BPA，酶解偶联，固相萃取和五氟苯溴衍生化。衍生化产物通过气相色谱分离，然后使用负化学电离和选择离子监测的质谱检测。使用这种分析方法，尿液样本中添加了固定水平的标记BPA和未标记BPA (0.27-10.6 ng/ml)，显示出恒定的回收率。此外，在一定范围内（0.25-10.0 ml）加入一定量的内标，产生线性响应（r(2)=0.99）。方法检出限为0.12 ng/ml。该方法通过气相色谱耦合高分辨率质谱仪的重复分析得到验证。
We report a new approach for assessing human exposure to bisphenol A (BPA) by measuring BPA in urine after enzymatic deglucuronidation. This method involves addition of (13)C(12)-labeled BPA, enzymatic deconjugation, solid-phase extraction, and derivatization with pentafluorobenzyl bromide. The product of the derivatization is separated by gas chromatography followed by mass spectrometric detection using negative chemical ionization and selected ion monitoring. Using this analysis method, urine samples fortified with both a constant level of labeled BPA and a range of unlabeled BPA levels (0.27-10.6 ng/ml) demonstrated constant percentage recovery. In addition, a range of urine sample volumes (0.25-10.0 ml) with constant amounts of added internal standard produced a linear response (r(2)=0.99). The method limit of detection was 0.12 ng/ml. This method was validated by duplicate analyses using gas chromatography coupled to a high-resolution mass spectrometer.