Generation, isolation, and maintenance of human mast cells and mast cell lines derived from peripheral blood or cord blood.
Generation, isolation, and maintenance of human mast cells and mast cell lines derived from peripheral blood or cord blood.
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DOI：
10.1002/0471142735.im0737s90
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发表时间：
2010-08-01
影响因子：
--
通讯作者：
Gilfillan, Alasdair M
中科院分区：
文献类型：
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作者：
Radinger, Madeleine;Jensen, Bettina M;Gilfillan, Alasdair M
抗原介导的肥大细胞活化是过敏性疾病（包括过敏反应和特应性反应）发生的关键步骤。迄今为止，旨在研究调节这些反应机制的研究，以及旨在确定预防这些反应的潜在方法的研究，主要在啮齿动物肥大细胞中进行。然而，要了解这些反应如何与人类疾病相关，并研究和开发治疗人类肥大细胞驱动疾病的新疗法，人类肥大细胞模型可能具有更大的相关性。最近，一些系统已经开发出来，使研究人员能够很容易地获得足够数量的人类肥大细胞来进行这些研究。这些肥大细胞释放适当的炎症介质，以响应已知的肥大细胞激活剂，包括抗原。这些系统也被用来检查调节这些反应的信号事件。原理研究的证明也证明了这些系统在识别肥大细胞激活和生长的潜在抑制剂方面的效用。在本单元中，描述了人类肥大细胞祖细胞的发育和培养技术以及人类肥大细胞系的培养技术。并介绍了每种模型的优缺点。
Antigen-mediated mast cell activation is a pivotal step in the initiation of allergic disorders including anaphylaxis and atopy. To date, studies aimed at investigating the mechanisms regulating these responses, and studies designed to identify potential ways to prevent them, have primarily been conducted in rodent mast cells. However, to understand how these responses pertain to human disease, and to investigate and develop novel therapies for the treatment of human mast cell-driven disease, human mast cell models may have greater relevance. Recently, a number of systems have been developed to allow investigators to readily obtain sufficient quantities of human mast cells to conduct these studies. These mast cells release the appropriate suite of inflammatory mediators in response to known mast cell activators including antigen. These systems have also been employed to examine the signaling events regulating these responses. Proof of principle studies has also demonstrated utility of these systems for the identification of potential inhibitors of mast cell activation and growth. In this unit, techniques for the development and culture of human mast cells from their progenitors and the culture of human mast cell lines are described. The relative merits and drawbacks of each model are also described.