Serum metabolomics analysis of mice that received repeated airway exposure to a water-soluble PM2.5 extract
Serum metabolomics analysis of mice that received repeated airway exposure to a water-soluble PM2.5 extract
复制标题
对反复气道暴露于水溶性 PM2.5 提取物的小鼠进行血清代谢组学分析
DOI：
10.1016/j.ecoenv.2018.10.068
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发表时间：
2019
影响因子：
6.8
通讯作者：
Wang Hongwei
中科院分区：
文献类型：
--
作者：
Zhao Chen;Niu Mengyuan;Song Shiyu;Li Jing;Su Zhonglan;Wang Yong;Gao Qian;Wang Hongwei
目的探讨大气颗粒物（PM2. 5）暴露对人体血清代谢物组学的影响，为PM2. 5暴露风险评估提供生物标志物。BALB/c小鼠接受急性或长期暴露于不溶性PM2.5颗粒或其水溶性提取物，并提交肺组织进行组织病理学分析。采用液相色谱/质谱联用仪（LC-MS）对PM2.5暴露前后的血清样品进行分析，结果可溶性PM2. 5样品中金属离子和无机离子含量最高。急性暴露于不溶性PM2.5颗粒和长期暴露于水溶性PM2.5提取物均引起严重的肺损伤，肺组织病理学评分与PM2.5暴露显著相关。代谢组学分析表明，长期暴露于水溶性PM2.5提取物与统计学显著的代谢物变化相关; 30种已知代谢物的血清浓度，包括磷脂、氨基酸和鞘脂的代谢物，在对照组和PM2.5暴露组之间存在显著差异。途径分析确定了三羧酸循环（TCA）和磷脂酶代谢途径与PM2.5暴露的关联。区分PM2.5暴露组血清和对照血清的最有影响的代谢物是LysoPE、LysoPC、LGPC、柠檬酸、PAF C-18、NeuAcalpha 2 -3Galbeta-Cer、Lyso-PAF C-16、神经节苷脂GA 2、1-sn-甘油-3-磷酸胆碱、PC和L-色氨酸。结论呼吸暴露于水-可溶性PM2.5提取物具有发育后果，不仅影响呼吸系统，而且影响新陈代谢。
BackgroundAir pollutant exposure negatively affects human health; however, the molecular mechanisms causing disease remain largely unclear.ObjectivesTo explore the effects of respiratory particulate matter (PM2.5) exposure on the serum metabolome and to identify biomarkers for risk assessment of PM2.5 exposure.MethodsPM2.5 from Nanjing, China, was collected, and its water-soluble extract was subjected to component analysis. BALB/c mice received acute or prolonged exposure to insoluble PM2.5 particles or its water-soluble extract, and lung tissue was submitted to histopathological analyses. Serum samples were collected pre- and post-PM2.5 exposure and analyzed by liquid chromatography/mass spectrometry.ResultsComponent analysis revealed that metals and inorganic ions were the most abundant components in the soluble PM2.5 samples. Acute exposure to insoluble PM2.5 particles and prolonged exposure to the water-soluble PM2.5 extract both induced severe lung injury, and the lung histopathological scores were significantly associated with PM2.5 exposure. Metabolomics analysis showed that prolonged exposure to the water-soluble PM2.5 extract was associated with statistically significant metabolite changes; the serum concentrations of 30 known metabolites, including metabolites of phospholipids, amino acids and sphingolipids, differed significantly between the control and PM2.5 exposure group. Pathway analysis identified an association of the tricarboxylic acid cycle (TCA) and the phospholipase metabolism pathway with PM2.5 exposure. The most influential metabolites for discriminating between the PM2.5-exposure group serum and the control serum were LysoPE, LysoPC, LGPC, citric acid, PAF C-18, NeuAcalpha2–3Galbeta-Cer, Lyso-PAF C-16, ganglioside GA2, 1-sn-glycero-3-phosphocholine, PC andL-tryptophan.ConclusionsRespiratory exposure to water-soluble PM2.5 extract has developmental consequences affecting not only the respiratory system but also metabolism.