Morphological and molecular phylogenetic analyses of Zepedanulus ishikawai (Arachnida: Opiliones: Laniatores: Epedanidae) in the southern part of the Ryukyu Archipelago
Morphological and molecular phylogenetic analyses of Zepedanulus ishikawai (Arachnida: Opiliones: Laniatores: Epedanidae) in the southern part of the Ryukyu Archipelago
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DOI：
10.4039/tce.2021.42
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发表时间：
2021-09
期刊：
影响因子：
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通讯作者：
Yoshimasa Kumekawa;Haruka Fujimoto;O. Miura;R. Arakawa;J. Yokoyama;T. Fukuda
中科院分区：
文献类型：
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作者：
Yoshimasa Kumekawa;Haruka Fujimoto;O. Miura;R. Arakawa;J. Yokoyama;T. Fukuda
摘要收割者是一种扩散能力极低的土壤动物，经历了异地分化。为了阐明琉球群岛南部特有的石川泽足蛛Zepedanulus ishikawai（Suzuki，1971）（Laniatores：Epedanidae）的形态学和系统发育分化，我们基于线粒体DNA的CO 1和16 S rRNA序列、nrDNA的28 S rRNA序列以及外部形态学进行了分子系统发育分析和分化时间估计。基于mtDNA序列构建的系统发育树表明，Z. Ishikawai为单系，可分为进化支I和进化支II。这一结果得到了nrDNA系统发育树的支持。虽然分支I和II共域分布在所有三个岛屿检查（石垣，西表，和与那国），异质性不能检测到聚合酶链反应-限制性片段长度多态性的nrDNA，表明分支I和II没有杂交的历史。此外，几个形态特征显着不同的个体之间的分支I和分支II。琉球南部与周围岛屿的长期隔离使Z的两个分支的原始形态特征的估计。石川。
Abstract Harvestmen (Arachnida: Opiliones) are soil animals with extremely low dispersal abilities that experienced allopatric differentiation. To clarify the morphological and phylogenetic differentiation of the endemic harvestman Zepedanulus ishikawai (Suzuki, 1971) (Laniatores: Epedanidae) in the southern part of the Ryukyu Archipelago, we conducted molecular phylogenetic analyses and divergence time estimates based on CO1 and 16S rRNA sequences of mtDNA, the 28S rRNA sequence of nrDNA, and the external morphology. A phylogenetic tree based on mtDNA sequences indicated that individuals of Z. ishikawai were monophyletic and were divided into clade I and clade II. This was supported by the nrDNA phylogenetic tree. Although clades I and II were distributed sympatrically on all three islands examined (Ishigaki, Iriomote, and Yonaguni), heterogeneity could not be detected by polymerase chain reaction–restriction fragment length polymorphism of nrDNA, indicating that clades I and II do not have a history of hybridisation. Also, several morphological characters differed significantly between individuals of clade I and clade II. The longstanding isolation of the southern Ryukyus from the surrounding islands enabled estimation of the original morphological characters of both clades of Z. ishikawai.