COMPLETE RECONSTITUTION OF MOUSE-LIVER WITH XENOGENEIC HEPATOCYTES
COMPLETE RECONSTITUTION OF MOUSE-LIVER WITH XENOGENEIC HEPATOCYTES
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DOI：
10.1073/pnas.92.11.4942
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发表时间：
1995-05-23
影响因子：
11.1
通讯作者：
BRINSTER, RL
中科院分区：
文献类型：
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作者：
RHIM, JA;SANDGREN, EP;BRINSTER, RL
我们已经开发了一种用于研究肝细胞生长潜力的系统，其中肝细胞被引入白蛋白尿激酶（Alb-uPA）转基因小鼠的患病肝脏中。将大鼠肝细胞导入免疫耐受的Alb-uPA转基因小鼠体内，研究异种细胞移植的可行性。在再生的受体肝脏中，高达100%的肝细胞基因表达是大鼠来源的，这表明产生了功能性小鼠肝脏，其中实质来源于异种（大鼠）肝细胞。免疫耐受性Alb-uPA转基因小鼠提供了一种在受控实验环境中研究任何物种（包括人类）肝细胞生物学的工具。
We have developed a system for studying hepatocellular growth potential in which liver cells are introduced into the diseased livers of albumin-urokinase (Alb-uPA) transgenic mice. To use this system to study xenogeneic cell transplantation, rat liver cells were introduced into immunotolerant Alb-uPA transgenic mice. In regenerated recipient livers, up to 100% of hepatocellular gene expression was of rat origin, demonstrating the creation of a functional mouse liver in which parenchyma is derived from xenogeneic (rat) hepatocytes. Immunotolerant Alb-uPA transgenic mice provide a tool for studying hepatocellular biology of any species, including humans, in a controlled experimental setting.