Imaging the functional organization of zebrafish hindbrain segments during escape behaviors
Imaging the functional organization of zebrafish hindbrain segments during escape behaviors
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DOI：
10.1016/s0896-6273(00)80246-9
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发表时间：
1996-12-01
期刊：
影响因子：
16.2
通讯作者：
Fetcho, JR
中科院分区：
文献类型：
--
作者：
OMalley, DM;Kao, YH;Fetcho, JR
虽然脊椎动物的后脑是分段的结构，但这种分段的功能意义尚不清楚。在斑马鱼中，后脑部分包含连续重复的可单独识别的神经元类别。我们利用斑马鱼幼鱼的透明性，并在完整的鱼中使用共聚焦钙成像来研究一组单独识别的连续同源网状脊髓细胞（Mauthner细胞，MiD2cm和MiD3cm）在行为过程中的活性。行为研究预测，这组连续同源神经元的差异活动可能有助于控制鱼类用来躲避捕食者的逃跑行为的方向性。我们发现，系列同源细胞确实是在逃逸过程中被激活的，并且激活的细胞的组合取决于用于引起逃逸的感觉刺激的位置。我们观察到的激活模式与行为研究所预测的完全一致。这些数据表明，祖先后脑部分的复制以及随后的功能多样化导致了相关神经元的活动模式产生行为变异。
Although vertebrate hindbrains are segmented structures, the functional significance of the segmentation is unknown. In zebrafish, the hindbrain segments contain serially repeated classes of individually identifiable neurons. We took advantage of the transparency of larval zebrafish and used confocal calcium imaging in the intact fish to study the activity of one set of individually identified, serially homologous reticulospinal cells (the Mauthner cell, MiD2cm, and MiD3cm) during behavior. Behavioral studies predicted that differential activity in this set of serially homologous neurons might serve to control the directionality of the escape behavior that fish use to avoid predators. We found that the serially homologous cells are indeed activated during escapes and that the combination of cells activated depends upon the location of the sensory stimulus used to elicit the escape. The patterns of activation we observed were exactly those predicted by behavioral studies. The data suggest that duplication of ancestral hindbrain segments, and subsequent functional diversification, resulted in sets of related neurons whose activity patterns create behavioral variability.