Differentiation of the gene clusters encoding botulinum neurotoxin type A complexes in Clostridium botulinum type A, Ab, and A(B) strains
Differentiation of the gene clusters encoding botulinum neurotoxin type A complexes in Clostridium botulinum type A, Ab, and A(B) strains
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DOI：
10.1128/aem.70.12.7192-7199.2004
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发表时间：
2004-12-01
影响因子：
4.4
通讯作者：
Aureli, P
中科院分区：
文献类型：
--
作者：
Franciosa, G;Floridi, F;Aureli, P
我们描述了一种策略，用于识别在意大利和美国从不同来源分离的 57 株 A、Ab 和 A(B) 型肉毒梭菌中编码 A 型肉毒杆菌毒素 (boNT/A) 复合物成分的基因簇。具体来说，我们将检测ha33和/或p47基因的PCR结果与boNT/A PCR-限制性片段长度多态性分析的结果结合起来。揭示了三种不同的A型毒素基因簇； A1 型在美国菌株中占主导地位，而 A2 型在意大利菌株中占主导地位，这表明菌株之间存在地理差异。相比之下，毒素基因簇与菌株的临床或食物来源之间没有明显的关系。在来自美国的两个 A 型肉毒杆菌分离株中，我们识别出第三个 A 型毒素基因簇（指定为 A3 型），该基因簇与之前仅针对 A(B) 型肉毒杆菌和 Ab 菌株描述的基因簇相似。对菌株总基因组DNA进行脉冲场凝胶电泳和随机扩增多态性DNA分析，结果与获得的boNT/A基因簇一致。
We describe a strategy to identify the clusters of genes encoding components of the botulinum toxin type A (boNT/A) complexes in 57 strains of Clostridium botulinum types A, Ab, and A(B) isolated in Italy and in the United States from different sources. Specifically, we combined the results of PCR for detecting the ha33 and/or p47 genes with those of boNT/A PCR-restriction fragment length polymorphism analysis. Three different type A toxin gene clusters were revealed; type A1 was predominant among the strains from the United States, whereas type A2 predominated among the Italian strains, suggesting a geographic distinction between strains. By contrast, no relationship between the toxin gene clusters and the clinical or food source of strains was evident. In two C. botulinum type A isolates from the United States, we recognized a third type A toxin gene cluster (designated type A3) which was similar to that previously described only for C. botulinum type A(B) and Ab strains. Total genomic DNA from the strains was subjected to pulsed-filed gel electrophoresis and randomly amplified polymorphic DNA analyses, and the results were consistent with the boNT/A gene clusters obtained.