Effects of maternal methyl donor on the pork characteristics of offspring pigs with prenatal exposure to bisphenol A
Effects of maternal methyl donor on the pork characteristics of offspring pigs with prenatal exposure to bisphenol A
复制标题
母体甲基供体对产前接触双酚A仔猪猪肉特性的影响
作者：
Y.Zhuo;J.Wang;H.Liu;D.Mou;L.Che;Z.Fang;S.Xu;G.Liu;Y.Lin;B.Feng;J.Li;D.Wu
为了探讨母体营养对后代肌肉特性的影响，将56头母猪在妊娠期间分配到四个饮食组之一：对照组（CON），或添加甲基供体（MET）、双酚A（BPA）和BPA和MET组合（BPA+MET）的对照饮食。与CON相比，MET后代的肉红度值较高，但胸最长肌（LT）的糖原含量较低。此外，与CON后代相比，MET后代在出生和肥育阶段表现出较低的LT糖原合成酶（GS）mRNA水平，并增加GS启动子的甲基化。产前BPA暴露降低了肉的pH值和红度值，但增加了LT肌肉的亮度值、乳酸含量、糖酵解潜力和乳酸脱氢酶（LDH）酶活性。出生前BPA暴露增加了出生时和完成阶段LT肌肉中LDH mRNA水平，并降低了LDH启动子的甲基化。因此，母体MET通过DNA甲基化影响肌肉GS和LDH表达，从而对猪肉品质产生持续影响。
To explore the effects of maternal nutrition on offspring muscle characteristics, a total of 56 sows were assigned to one of the four dietary groups during gestation: control (CON), or control diets supplemented with methyl donor (MET), bisphenol A (BPA), and combined BPA and MET (BPA+MET). Compared with CON offspring, MET offspring showed a higher meat redness value, but lower glycogen content in the longissimus thoracis (LT). Moreover, compared with CON offspring, MET offspring showed lower LT glycogen synthase (GS) mRNA levels at birth and the finishing stage, and increased methylation at the GS promoter. Prenatal BPA exposure reduced the pH and redness value of meat, but increased the lightness value, lactate content, glycolytic potential and lactate dehydrogenase (LDH) enzyme activity in the LT muscle. Prenatal BPA exposure increased LDH mRNA levels in the LT muscle at birth and the finishing stage, and reduced methylation at the LDH promoter. Thus, maternal MET affects muscle GS and LDH expression via DNA methylation, thereby resulting in persistent effects on pork quality.