Histopathological effects of waterborne copper nanoparticles and copper sulphate on the organs of rainbow trout (Oncorhynchus mykiss)
Histopathological effects of waterborne copper nanoparticles and copper sulphate on the organs of rainbow trout (Oncorhynchus mykiss)
复制标题
DOI：
10.1016/j.aquatox.2012.10.005
复制
发表时间：
2013-01-15
影响因子：
4.5
通讯作者：
Henry, Theodore B.
中科院分区：
文献类型：
--
作者：
Al-Bairuty, Genan A.;Shaw, Benjamin J.;Henry, Theodore B.
目前还不清楚铜纳米颗粒是否比传统形式的溶解铜更具毒性。本研究旨在描述幼年虹鳟鱼（Oncorhynchus mykiss）的鳃、肠、肝、肾、脑和肌肉的病理学，虹鳟鱼在半静态水性暴露方案中暴露于对照（未添加Cu）、20或100 μ g（-1）溶解的Cu（以CuSO 4计）或Cu-NPs（平均初级颗粒尺寸为87 +/-27 nm）。在第0、4和10天对鱼取样进行组织学分析。所有处理均引起器官损伤，并且用Cu-NP观察到的病理类型与CuSO4大致相同，包括：鳃的次级层中的增生、变性和坏死;杯状细胞肿胀、粘膜层中的坏死和肠中的空泡形成;肝中的肝炎样损伤和具有固缩核的细胞;一些肾小管上皮细胞受损，肾脏Bowman氏间隙增加。在脑中，观察到端脑神经细胞体的一些轻度变化、中脑层厚度的改变和小脑腹面血管的扩大。在骨骼肌中观察到肌纤维面积比例的变化。总的来说，数据显示，从CuSO4和Cu-NPs的病理是相似的类型，但有一些材料类型的影响，在严重程度或发生率的伤害与Cu-NPs造成更多的伤害，在肠道，肝脏和大脑比同等浓度的CuSO4的实验结束时，但在鳃和肌肉的CuSO4造成更多的病理。(c)2012爱思唯尔有限公司版权所有。
It is unclear whether copper nanoparticles are more toxic than traditional forms of dissolved copper. This study aimed to describe the pathologies in gill, gut, liver, kidney, brain and muscle of juvenile rainbow trout, Oncorhynchus mykiss, exposed in triplicate to either a control (no added Cu), 20 or 100 mu gl(-1) of either dissolved Cu (as CuSO4) or Cu-NPs (mean primary particle size of 87 +/- 27 nm) in a semi-static waterborne exposure regime. Fish were sampled at days 0, 4, and 10 for histology. All treatments caused organ injuries, and the kinds of pathologies observed with Cu-NPs were broadly of the same type as CuSO4 including: hyperplasia, aneurisms, and necrosis in the secondary lamellae of the gills; swelling of goblet cells, necrosis in the mucosa layer and vacuole formation in the gut; hepatitis-like injury and cells with pyknotic nuclei in the liver; damage to the epithelium of some renal tubules and increased Bowman's space in the kidney. In the brain, some mild changes were observed in the nerve cell bodies in the telencephalon, alteration in the thickness of the mesencephalon layers, and enlargement of blood vessel on the ventral surface of the cerebellum. Changes in the proportional area of muscle fibres were observed in skeletal muscle. Overall the data showed that pathology from CuSO4 and Cu-NPs were of similar types, but there were some material-type effects in the severity or incidence of injuries with Cu-NPs causing more injury in the intestine, liver and brain than the equivalent concentration of CuSO4 by the end of the experiment, but in the gill and muscle CuSO4 caused more pathology. (c) 2012 Elsevier B.V. All rights reserved.