A novel post-developmental role of the Hox genes underlies normal adult behaviour
A novel post-developmental role of the Hox genes underlies normal adult behaviour
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Hox 基因的一种新的发育后作用是正常成人行为的基础
DOI：
10.1101/2021.08.29.458086
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发表时间：
2021
期刊：
影响因子：
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通讯作者：
Raouf Issa A
中科院分区：
文献类型：
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作者：
Raouf Issa A
成熟神经元和神经回路稳定性的分子机制尚不清楚。在这里，我们探索这个问题，并发现hoxgenes是维持成年果蝇正常神经功能的遗传程序的一个组成部分。我们发现，成年神经元中hoxgeneultrabithorax （Ubx）的发育后下调会导致飞行中的实质性异常。绘制这些效应的细胞基础揭示了ubxis在多巴胺能神经元的一个子集中是必需的，细胞电路分析和光遗传学使我们能够将这些多巴胺能神经元与飞行控制联系起来。功能成像实验表明ubxx对于正常的多巴胺能活动是必要的，神经元特异性rna测序确定了两个以前未被表征的离子通道编码基因作为fuxx行为作用的潜在介质。因此，我们的研究揭示了hox系统在控制成人行为和神经功能方面的新作用。
The molecular mechanisms underlying the stability of mature neurons and neural circuits are poorly understood. Here we explore this problem and discover that theHoxgenes are a component of the genetic programme that maintains normal neural function in adultDrosophila. We show that post-developmental downregulation of theHoxgeneUltrabithorax(Ubx) in adult neurons leads to substantial anomalies in flight. Mapping the cellular basis of these effects reveals thatUbxis required within a subset of dopaminergic neurons, and cell circuitry analyses and optogenetics allow us to link these dopaminergic neurons to flight control. Functional imaging experiments show thatUbxis necessary for normal dopaminergic activity, and neuron-specific RNA-sequencing defines two previously uncharacterised ion channel-encoding genes as potential mediators ofUbxbehavioural roles. Our study thus reveals a novel role of theHoxsystem in controlling adult behaviour and neural function.