Characterization of potential regulatory elements within the rat arginine vasopressin proximal promoter
Characterization of potential regulatory elements within the rat arginine vasopressin proximal promoter
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DOI：
10.1054/npep.1999.0018
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发表时间：
1999-02-01
期刊：
影响因子：
2.9
通讯作者：
Quinn, JP
中科院分区：
文献类型：
--
作者：
Grace, CO;Fink, G;Quinn, JP
为了确定潜在的顺式作用的调节元件在大鼠精氨酸加压素启动子，跨越核苷酸-374到-36，我们进行了DNA酶1足迹分析，电泳迁移率变动分析补充。该分析已经在该区域内鉴定了不同的转录因子结合位点。这些是：1）五个E盒基序，其被转录因子的基本螺旋环螺旋家族的成员结合; 2）两个富含dGC的元件，其结合包括Spl的多个复合物; 3）单个AP 1元件，其结合含有c-Fos或Fos相关抗原的复合物。这些元素的位置和它们之间的相互关系表明这些因素之间的组合相互作用作为大鼠精氨酸加压素近端启动子的转录调控机制。
In order to identify potential cis acting regulatory elements in the rat arginine vasopressin promoter, spanning nucleotides -374 to -36, we have carried out DNAase1 footprint analysis complemented by electrophoretic mobility shift analysis. This analysis has identified distinct transcription factor binding sires within this region. These are: 1) five E box motifs which are bound by members of the basic helix loop helix family of transcription factors; 2) two dGC rich elements which bind multiple complexes including Spl; 3) a single AP1 element which binds a complex containing c-Fos or Fos related antigens. The location of these elements and their relationship to one another suggests combinatorial interaction amongst these factors as a mechanism of transcriptional regulation of the rat arginine vasopressin proximal promoter.