Melanoma Regression and Recurrence in Zebrafish.
Melanoma Regression and Recurrence in Zebrafish.
复制标题
DOI：
10.1007/978-1-4939-3771-4_10
复制
发表时间：
2016
影响因子：
--
通讯作者：
S. Wojciechowska;Zhiqiang Zeng;J. Lister;C. Ceol;E. Patton
中科院分区：
文献类型：
--
作者：
S. Wojciechowska;Zhiqiang Zeng;J. Lister;C. Ceol;E. Patton
黑色素瘤是最致命的皮肤癌，死亡率很高。大多数黑色素瘤患者存在BRAF(V600E)的激活突变，BRAFV600E的选择性抑制剂已成功应用于患者。然而，在最初的肿瘤消退后，大多数患者会产生耐药性，导致肿瘤再生长。因此，重要的是要了解这些进程背后的机制。我们最近描述了主要的黑素细胞转录因子MITF在肿瘤生长、消退和复发中的作用。在这里，我们描述了使用人类黑色素瘤的温度敏感型斑马鱼模型来研究体内退化和复发以及组织学和免疫组织化学的方案。
Melanoma is the most lethal form of skin cancer with high mortality rates. Most melanoma cases have activating mutations in BRAF (V600E) and the selective inhibitors of BRAFV600Ehave been successfully used in patients. However, after initial tumor regression, the majority of patients develop drug resistance resulting in tumor regrowth. It is therefore important to understand the mechanisms underlying these processes. We have recently described the role of the master melanocyte transcription factor MITF in tumor growth, regression, and recurrence. Here, we describe protocols to study regression and recurrence in vivo, as well as for histology and immunohistochemistry, using a temperature-sensitive zebrafish model of human melanoma.