Cell type-dependent localization of MLO proteins
Cell type-dependent localization of MLO proteins
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MLO 蛋白的细胞类型依赖性定位
DOI：
10.1080/15592324.2017.1393135
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发表时间：
2017
影响因子：
2.9
通讯作者：
Kessler, Sharon A.
中科院分区：
文献类型：
--
作者：
Jones, Daniel S.;Kessler, Sharon A.
抗霉基因座O（MLO）蛋白是植物中介导细胞间通讯的跨膜蛋白。我们最近证明了亚细胞定位的重要性，MLO功能在花粉管接收。NORTIA（NTA）是参与这一过程的MLO蛋白，在与花粉管相互作用之前定位于助细胞的高尔基体。MLO蛋白，可以取代NTA的功能，在这一途径中都部分定位与相同的高尔基体标记助细胞。在这项研究中，我们报告说，MLO亚细胞定位是细胞类型依赖性的，与不同的分布时观察到的一些MLO异位表达在烟草和拟南芥的表皮细胞相比，助细胞。这种依赖性可能是由于在给定的细胞类型内影响MLO功能的辅因子，为理解MLO分布及其各自过程中的后续功能提供了一个有趣的新目标。
Mildew resistance locus O (MLO) proteins are transmembrane proteins that mediate cell-cell communication in plants. We recently demonstrated the importance of subcellular localization to MLO function during pollen tube reception. NORTIA (NTA), the MLO protein involved in this process, localizes to the Golgi of the synergid cell before interaction with the pollen tube. MLO proteins that can substitute for NTA's function in this pathway all partially localize with the same Golgi marker in the synergid cell. In this study, we report that MLO subcellular localization is cell type-dependent, with different distributions of some MLOs observed when ectopically expressed in the epidermal cells of tobacco and Arabidopsis compared to synergids. This dependency may be due to co-factors that influence MLO function within a given cell type, providing an intriguing new target for understanding MLO distribution and subsequent function in their respective processes.