Genetical metabolomics of flavonoid biosynthesis in Populus:: a case study
Genetical metabolomics of flavonoid biosynthesis in Populus:: a case study
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DOI：
10.1111/j.1365-313x.2006.02786.x
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发表时间：
2006-07-01
期刊：
影响因子：
7.2
通讯作者：
Boerjan, Wout
中科院分区：
文献类型：
--
作者：
Morreel, Kris;Goeminne, Geert;Boerjan, Wout
遗传代谢组学[代谢物分析与数量性状位点（QTL）分析相结合]已被提出作为一种新的工具，以确定控制代谢物丰度的基因座。这一概念进行了评估，在一个案例研究与模式树杨树。采用高效液相色谱法，分析了15个密切相关的黄酮类化合物在两个全同胞白杨家庭，美洲黑杨cv。S9-2 x黑胡椒品种。Ghoy和美洲黑杨P. deltoides cv. S9-2 ×大果杨V24，相关分析和QTL分析，检测类黄酮生物合成的通量控制点。四个强大的代谢物数量性状位点（mQTL），与类黄酮生物合成的限速步骤，映射。每个mQTL都参与对一种或两种黄酮类化合物的通量控制。基于受影响的代谢产物的身份和类黄酮途径的结构，一个试探性的功能分配给这些mQTL中的三个，并映射相应的候选基因。这些数据表明，代谢产物分析与QTL分析的组合是一种有价值的工具，以确定控制点的密切相关的化合物的复杂代谢途径。
Genetical metabolomics [metabolite profiling combined with quantitative trait locus (QTL) analysis] has been proposed as a new tool to identify loci that control metabolite abundances. This concept was evaluated in a case study with the model tree Populus. Using HPLC, the peak abundances were analyzed of 15 closely related flavonoids present in apical tissues of two full-sib poplar families, Populus deltoides cv. S9-2 x P. nigra cv. Ghoy and P. deltoides cv. S9-2 x P. trichocarpa cv. V24, and correlation and QTL analysis were used to detect flux control points in flavonoid biosynthesis. Four robust metabolite quantitative trait loci (mQTL), associated with rate-limiting steps in flavonoid biosynthesis, were mapped. Each mQTL was involved in the flux control to one or two flavonoids. Based on the identities of the affected metabolites and the flavonoid pathway structure, a tentative function was assigned to three of these mQTL, and the corresponding candidate genes were mapped. The data indicate that the combination of metabolite profiling with QTL analysis is a valuable tool to identify control points in a complex metabolic pathway of closely related compounds.