GPR37 and GPR37L1 differently interact with dopamine 2 receptors in live cells.
GPR37 and GPR37L1 differently interact with dopamine 2 receptors in live cells.
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DOI：
10.1016/j.neuropharm.2018.11.009
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发表时间：
2019-07-01
影响因子：
4.7
通讯作者：
Svenningsson P
中科院分区：
文献类型：
--
作者：
Hertz E;Terenius L;Vukojević V;Svenningsson P
受体-受体相互作用对于精细调节受体反应是必不可少的，并且新技术能够更密切地表征直接在质膜中所涉及的蛋白质之间的相互作用。荧光互相关光谱（FCCS），分析结合的分子与单分子检测限的同时运动，在这里被用来，在活的N2 a细胞，研究帕金森病（PD）相关的孤儿受体GPR 37，其同系物GPR 37 L1，和多巴胺2受体（D2 R）的两个剪接变体之间的相互作用。检测到GPR 37与D2 R的两种剪接形式之间的相互作用。4-苯丁酸（4-PBA），一种已知增加细胞表面GPR 37表达的神经保护性化学伴侣，增加了相互作用分子的分数。普拉克索（一种常用于治疗PD的D2 R激动剂）也增加了相互作用，表明可能具有临床意义。还检测到指示GPR 37 L1与D2 R的短同种型之间的相互作用的交叉相关。然而，这种相互作用在4-PBA或普拉克索治疗后没有改变。总体而言，这些数据提供了异聚体GPR 37-D2 R存在并且可以被调节的进一步证据，这与PD的治疗相关。这篇文章是特刊的一部分，题为“受体异聚体和它们的变构受体-受体相互作用”。用荧光互相关光谱研究了GPCR相互作用。在活细胞中检测到GPR 37与D2 R的两种同种型之间的相互作用。在活细胞中检测到GPR 37的同源物GPR 37 L1与D2 RS相互作用。GPR 37-D2 R相互作用通过D2样激动剂和4-PBA处理而增加。
Receptor-receptor interactions are essential to fine tune receptor responses and new techniques enable closer characterization of the interactions between involved proteins directly in the plasma membrane. Fluorescence cross-correlation spectroscopy (FCCS), which analyses concurrent movement of bound molecules with single-molecule detection limit, was here used to, in live N2a cells, study interactions between the Parkinson's disease (PD) associated orphan receptor GPR37, its homologue GPR37L1, and the two splice variants of the dopamine 2 receptor (D2R). An interaction between GPR37 and both splice forms of D2R was detected. 4-phenylbutyrate (4-PBA), a neuroprotective chemical chaperone known to increase GPR37 expression at the cell surface, increased the fraction of interacting molecules. The interaction was also increased by pramipexole, a D2R agonist commonly used in the treatment of PD, indicating a possible clinically relevance. Cross-correlation, indicating interaction between GPR37L1 and the short isoform of D2R, was also detected. However, this interaction was not changed with 4-PBA or pramipexole treatment. Overall, these data provide further evidence that heteromeric GPR37-D2R exist and can be pharmacologically modulated, which is relevant for the treatment of PD. This article is part of the Special Issue entitled ‘Receptor heteromers and their allosteric receptor-receptor interactions’. GPCR interaction is studied with fluorescence cross-correlation spectroscopy. Interaction between GPR37 and both isoforms of D2R is detected in live cells. GPR37's homologue GPR37L1 is detected to interact with D2RS in live cells. GPR37-D2R interaction is increased by D2-like agonist and 4-PBA treatment.
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影响因子：
5.3
作者：
Borroto-Escuela DO;Carlsson J;Ambrogini P;Narváez M;Wydra K;Tarakanov AO;Li X;Millón C;Ferraro L;Cuppini R;Tanganelli S;Liu F;Filip M;Diaz-Cabiale Z;Fuxe K
通讯作者：
Fuxe K
帕金森氏病相关的GPR37受体与纹状体腺苷A（2a）受体相互作用，该受体控制其细胞表面表达和体内功能。
影响因子：
4.6
作者：
Morató X;Luján R;López-Cano M;Gandía J;Stagljar I;Watanabe M;Cunha RA;Fernández-Dueñas V;Ciruela F
通讯作者：
Ciruela F
影响因子：
64.5
作者：
Hauser AS;Chavali S;Masuho I;Jahn LJ;Martemyanov KA;Gloriam DE;Babu MM
通讯作者：
Babu MM
影响因子：
4.7
作者：
Gu, M;Irvani, M;Schapira, AHV
通讯作者：
Schapira, AHV
影响因子：
3.5
作者：
Kitao, Yasuko;Imai, Yuzuru;Ogawa, Satoshi
通讯作者：
Ogawa, Satoshi