Bioassay of Clostera anastomosis granulosis virus
Bioassay of Clostera anastomosis granulosis virus
复制标题
DOI：
10.1007/s11676-006-0012-6
复制
发表时间：
2006-03
影响因子：
3
通讯作者：
Li Haixia;W. Zhiying;Guo Shuping;Xie Shuping
中科院分区：
文献类型：
--
作者：
Li Haixia;W. Zhiying;Guo Shuping;Xie Shuping
在黑龙江省双城镇杨树人工林中采集了吻合螟二龄健康幼虫。死幼虫。对感染吻合口菌颗粒病病毒（GV）的吻合口进行研磨，获得GV。将GV病毒农药稀释至1.58×103PIB·mL−1、1.58×104PIB·mL−1、1.58×105PIB·mL−1、1.58×106PIB·mL−1、1.58×107PIB·mL−1、1.58×108PIB·mL−1和1.58×109PIB·mL−1 7种浓度的液体，将新鲜杨叶浸于这7种浓度的液体中饲喂幼虫。9 d后观察幼虫的死亡率。1.58×103PIB·mL−1处理的校正死亡率最低（7.32%），1.58×109PIB·mL−1处理的校正死亡率最高（97.36%）。病毒浓度的对数与死亡率的回归方程为y=1.946+0.558x。lc50为2.97×105PIB·mL−1。病毒浓度1.58×105、1.58×106、1.58×107、1.58×108、1.58×109PIB·mL−1的lt50分别为8.55d、6.89d、5.9d、4.65d和4.08d，随着浓度的增加，lt50逐渐缩短。综上所述，吻合梭蝇的毒性很强，作为一种杀虫剂具有很大的实际应用潜力。
The second-instar healthy larvae ofClostera anastomosiswere collected in the artificial woodland of poplar in Shuangcheng Town, Heilongjiang Province, China. The dead larvae ofC. anastomosisinfected by granulosis virus (GV) ofClostera anastomosiswere grinded to obtain GV. The GV viral pesticide was diluted to seven concentrations, 1.58×103PIB·mL−1, 1.58×104PIB·mL−1, 1.58×105PIB·mL−1, 1.58×106PIB·mL−1, 1.58×107PIB·mL−1, 1.58×108PIB·mL−1and 1.58×109PIB·mL−1and the fresh poplar leaves were dipped in the seven concentrations liquids to feed the larvae. After nine days the mortality of larvae was investigated. The minimum corrected mortality (7.32%) of larvae was observed at concentrations of 1.58×103PIB·mL−1and the maximal mortality (97.36%) was observed at concentration of 1.58×109PIB·mL−1. The regression equation between the logarithm of the virus concentration and the mortality was y=1.946+0.558x. The LC50was 2.97×105PIB·mL−1. The LT50for the virus concentration of 1.58×105, 1.58×106, 1.58×107, 1.58×108, 1.58×109PIB·mL−1were 8.55d, 6.89d, 5.9d, 4.65d, and 4.08d, respectively, shorting gradually with the concentration increasing. It is concluded that the toxicity ofClostera anastomosisGV is very strong and as a kind of insecticides it has big potential in practical application.